Microporation as a new efficient approach for transfection

微孔化作为一种​​新的有效转染方法

• 批准号: 360398-2008
• 负责人: Barbeau, Benoit
• 金额: $ 1.53万
• 依托单位: Université du Québec à Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=362626
• 关键词: Microporation; new; efficient; approach; transfection

In this proposal, we are requesting the purchase of the Microporator MP-100 transfection system.  Based on preliminary data from both of our research group, this device should permit to introduce DNA or RNA molecules in an efficient manner in cells, which are refractory to such a procedure.  Numerous advantages can be cited to describe this apparatus such as its easy-to-use characteristic, its small size, its flexibility to different cells, etc.  This apparatus will be very beneficial to both B. Barbeau and C. Mounier's laboratories and will be used for projects related to the study of placenta development and hormone- and nutrient-based regulation of FAS and SCD1 enzymes.  These studies indeed involve human primary cells known as cytotrophoblast cells (placenta) and chicken embryo hepatocytes.  These cells are thereby difficult to easily transfer nucleic acids internally and early results from our laboratory suggest that the Microporator indeed provides the needed technology to achieve this goal in both cell context.  We believe that the success of these projects will therefore be assured by the acquisition of the Microporator allowing us to test our hypotheses in more physiologically relevant cell systems.  The data that will emanate from these experiments will greatly contribute in the understanding of the process of placenta development and gene regulation of liver enzymes.  However, these results also has a strong potential to benefit Canadians as the pathologies/disorders which are related to our research themes are important health issues in Canada, such as preeclampsia and other pregnancy-related disorders for the cytotrophoblast project and cancer and obesity for the studied FAS/SCD1 genes.

在此提案中，我们要求购买Microporator MP-100转染系统。根据我们两个研究小组的初步数据，该装置应该可以有效地将DNA或RNA分子引入细胞中，这是一种难以采用这种方法的方法。许多优点可以被引用来描述这种装置，如它易于使用的特点，它的小尺寸，它的灵活性，不同的细胞等。该仪器将非常有利于B. Barbeau和C. Mounier的实验室，并将用于研究胎盘发育和基于激素和营养的FAS和SCD1酶调节的相关项目。这些研究确实涉及被称为细胞滋养层细胞（胎盘）的人类原代细胞和鸡胚肝细胞。因此，这些细胞很难轻易地在内部转移核酸，我们实验室的早期结果表明，Microporator确实提供了在两种细胞环境中实现这一目标所需的技术。我们认为，这些项目的成功将因此获得Microporator的保证，使我们能够在生理上更相关的细胞系统中测试我们的假设。从这些实验中获得的数据将大大有助于了解胎盘发育过程和肝酶的基因调控。然而，这些结果也有很强的潜力使加拿大人受益，因为与我们的研究主题相关的病理/疾病是加拿大重要的健康问题，例如细胞滋养细胞项目的先兆子痫和其他妊娠相关疾病，以及研究的FAS/SCD1基因的癌症和肥胖。