DNA analysis infrastructure

我的研究计划的目标是：(1)记录自然种群内部和之间的进化分化模式；(2)检验关于生物多样性从独特种群到物种起源的假设；(3)将这种理解应用于生物多样性的保护。用于解决进化生物学中这些关键问题的模型是北美西北部的鱼类，包括：在不同的冰川避难所中分离出来的进化谱系（棘鱼、鳟鱼和鲑鱼）；通过自然地貌（例如，鳟鱼、鲑和灰鲑的迁移障碍、流域互联性）或生态（鳟鱼和棘鱼对不同栖息地的适应）表现出不同程度物理分离的种群；以及冰期后接触到的近亲鱼类（棘鱼和棘鱼）。该研究项目的新颖之处包括：整合历史和当代的分化过程，对这些过程在旧的（如char）和最近的（如棘鱼）分化中的比较分析，以及在假设检验中整合分子和生态方法。我们的工作对培养加拿大急需的水生和分子生态学以及生物多样性研究领域的年轻科学家非常重要。这项研究计划的基础是对从北美西北部收集的鱼类中获得的DNA进行分析。该提案寻求资金翻新老化，但对提取和初步操作DNA至关重要的设备。

The goals of my research program are to: (i) document the patterns of evolutionary divergence within and among natural populations, (ii) test hypotheses concerning the origins of biodiversity from unique populations to species, and (iii) apply such understanding to the conservation of biodiversity. The models used to address these key questions in evolutionary biology are fishes of northwestern North America, including: evolutionary lineages that were isolated in distinct glacial refugia (sticklebacks, trout, and char); populations that show various degrees of physical separation via physical landform (e.g., migration barriers, watershed interconnectedness in trout, char, and grayling) or ecology (adaptation to distinct habitats in trout and sticklebacks); and species of closely related fishes that have come into contact (char and sticklebacks) postglacially. The novel aspects of this research program include: integration of historical and contemporary processes in divergence, comparative analyses of these processes in old (e.g., char) and more recent (e.g., sticklebacks) divergences, and the integration of molecular andecological methods in hypothesis testing.  Our work is important for the training of young scientists in areas of great need for Canada - aquatic and molecular ecology and biodiversity research.  Fundamental to this research program is the analysis of DNA obtained from fishes collected from throughout northwestern North America. This proposal seeks funds to refurbish aging, but essential equipment central to the extraction and initial manipulation of DNA.