Digital imaging of developing organisms

了解生物体如何发育是了解生物体如何发挥功能的关键一步。 了解动物发育的一个关键方面需要能够操纵基因或蛋白质表达，然后测试对胚胎或动物发育的影响。 这可以通过一系列方式来完成，包括使用基因突变或破坏蛋白质生产或改变发育信号发生方式的方法。 然而，无论要研究的生物体或破坏发育过程的方式如何，可视化对胚胎发育的影响的能力都是这种方法的重要组成部分。 对于这些方法，需要对发育中的生物体或组织进行成像。 研究人员和他们的实验室需要这种设备来研究开发的不同方面。 然而，他们都使用类似的方法，利用抗体与组织染色配对的发育调节蛋白质，以评估蛋白质表达，信号传导，细胞命运或形态的变化。 因此，高分辨率显微镜是所有发育生物学实验室的重要组成部分。 分析的许多发育缺陷都是在单细胞或发育组织的尺度上，因此使用了高放大率与优秀的光学和镜头。 与这些高品质显微镜的使用配对的是需要捕捉这些图像，以进一步分析和向该领域的其他人展示数据。 这需要一个高端数码相机与计算机和软件配对，以符文的相机，已在此授权申请要求。 要求数码相机能够以高分辨率捕获大量数据，以忠实地表示通过显微镜观察到的数据。 此外，数码相机需要一台计算机，该计算机具有必要的软件来运行相机，存储捕获的数据并分析生成的图像。

Understanding how an organism develops is a critical step in understanding how an organism functions.  One of the key apsects of understanding animal developmen requires the ability to manuipulate gene or protein expression and then test the effects on embryonic or animal development.  This can be done in a range of ways including the use of genetic mutations or methods that disrupt protein production or change how developmental signaling occurs.  However, no matter the organism to be investigated or the manner of disrupting developmental processes, the ability to visualize the effects on embryonic development is a vital part of this approach.  For these approaches imaging of developing organisms or tissues is required.  The researchers and their laboratories requesting this equipment study different aspects of development.  However they all use similar approaches that make use of antibodies to developmentally regulated  proteins paired with tissue staining to assess changes in protein expression, signaling, cell fate or morphology.  For this high resolution microscopes are an essential part of all developmental biology laboratories.  Many of the developmental defects analyzed are on the scale of single cells or developing tissues and thus high magnification paired with excellent optics and lenses are used.  Paired with the use of these high quality microscopes is the need to capture these images for futher analysis and presentation of the data to others in the field.   This requires a high end digital camera paired with a computer and software to rune the camera that has been requested in this grant applicaiton.  A digital camera has been requested that can capture large amounts of data with high resolution to faithfully represent the data observed through the microscope.  In addition the digital camera requires a computer with the necessary software to run the camera, store the captured data and analyze the generated images.