Single Molecule Cell Biology

单分子细胞生物学

• 批准号: nhmrc : 433624
• 负责人: A/Pr Andrew Clayton
• 金额: $ 19.81万
• 依托单位: Swinburne University of Technology
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2007
• 资助国家: 澳大利亚
• 起止时间: 2007-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/single-molecule-cell-biology/98260
• 关键词: Single; Molecule; Cell; Biology

Our current understanding of cellular signalling and disease is based on ensemble measurements over a cellular or molecular population. While these measurements have provided valuable information on the molecular circuitry required for cellular function, there is a lack of detail on the spatio-temporal dynamics of signal initiation and propagation at the single molecule and single cellular level. Single particle (molecule or cell) approaches offer the advantage of being able to detect individual processes including rare events that would be lost in an ensemble measurement. Moreover single particle approaches provide dynamic-kinetic information that does not rely on synchronising a population of molecules or cells. In this proposal we aim to build on our combined expertise in EGF-EGFR signalling, biophysics, biosensors, quantum dot nanotechnology and single molecule spectroscopy to learn more about how EGFR cellular signalling works and how it is impaired in cancer. This project will provide basic information that could lead to the design of more effective drugs directed agaisnt this therapeutic target.

我们目前对细胞信号传导和疾病的理解是基于对细胞或分子群体的整体测量。虽然这些测量提供了细胞功能所需的分子电路的有价值的信息，但缺乏在单分子和单细胞水平上的信号启动和传播的时空动力学的细节。单粒子（分子或细胞）方法的优点是能够检测单个过程，包括在整体测量中丢失的罕见事件。此外，单粒子方法提供了不依赖于同步分子或细胞群体的动态-动力学信息。在这项提案中，我们的目标是建立在我们在EGF-EGFR信号传导，生物物理学，生物传感器，量子点纳米技术和单分子光谱学方面的综合专业知识的基础上，更多地了解EGFR细胞信号传导如何工作以及它如何在癌症中受损。该项目将提供基本信息，可能导致设计更有效的药物针对这一治疗目标。