Fluorescence analysis of the EGFreceptor signalling network

EG受体信号网络的荧光分析

• 批准号: nhmrc : 280918
• 负责人: A/Pr Andrew Clayton
• 金额: $ 32.72万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2004
• 资助国家: 澳大利亚
• 起止时间: 2004-01-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/fluorescence-analysis-egfreceptor-signalling-network/82786
• 关键词: Fluorescence; analysis; EGFreceptor; signalling; network

Receptors are cell-surface molecules that enable the cell to receive chemical messages from the outside environment and transmit these signals to the inside of cell. These messages tell the cells to grow, divide or die. The Epidermal Growth Factor Receptor is linked to a variety of cell signalling pathways that are critical to the normal functioning of cells. Conversely, abberations in Epidermal Growth Factor-mediated cell signalling leads to many types of cancers. A basic understanding of how the receptor is turned off or on is essential to designing drugs that can specifically inhibit its hyperproliferative response. High resolution structures of a key part of the Epidermal Growth Factor Receptor have identified several structural forms of the receptor that are providing valuable clues as to the structural basis for receptor activation. Armed with this information and advanced microscopic imaging technology we are in the unique position to probe receptor activation in living cells. This project seeks to determine which structural form of the receptor is responsible for transmission of cellular messages and how it is impaired in cancerous cells.

受体是细胞表面分子，使细胞能够从外部环境接收化学信息并将这些信号传输到细胞内部。这些信息告诉细胞生长、分裂或死亡。表皮生长因子受体与多种细胞信号传导途径有关，这些信号传导途径对细胞的正常功能至关重要。相反，表皮生长因子介导的细胞信号传导异常会导致多种类型的癌症。对受体如何关闭或开启的基本了解对于设计能够特异性抑制其过度增殖反应的药物至关重要。表皮生长因子受体关键部分的高分辨率结构已经确定了受体的几种结构形式，为受体激活的结构基础提供了有价值的线索。凭借这些信息和先进的显微成像技术，我们在探测活细胞中的受体激活方面处于独特的地位。该项目旨在确定受体的哪种结构形式负责细胞信息的传输以及它在癌细胞中如何受损。