Surface plasmon resonance instrumentation for characterization of biomolecular interactions

生物分子间的相互作用是细胞和组织功能的核心。这项建议的目的是在物理系和生物系获得用于研究生物分子相互作用的表面等离子体共振(SPR)仪器。渥太华生物科学界的主要研究人员和其他跨学科研究人员的研究计划需要定量描述受体-配体结合过程的手段，这些过程管理(1)生物分子组成的复合体的组装，以及(2)细胞与细胞外环境中生物大分子之间的相互作用。所要求的SensiQ生物分子相互作用系统是一种基于流体的多功能双通道SPR传感系统，可用于确定生物大分子或完整细胞与显示的分子受体结合的平衡受体-配体结合亲和力和动力学常数。除了对我们的具体研究计划产生重要影响外，收购这一SPR系统还将加强生物物理、分子生物学、环境基因组学高级研究中心(CAREG)和新成立的生物材料研究中心的跨学科研究和培训工作，并将为HQP在分子生物物理、细胞生物学和定量生物医学科学等一般领域的培训提供重要资源。

Biomolecular interactions are central to the function of cells and tissues. The aim of this proposal is to acquire surface plasmon resonance (SPR) instrumentation for the study of biomolecular interactions in the Departments of Physics and Biology. Research programs of the principal investigators and others in the interdisciplinary uOttawa biological sciences community require the means for quantitative characterization of receptor-ligand binding processes that govern (1) the assembly of complexes of biomolecular components, and (2) the interaction between cells and biomacromolecules in their extracellular environment. The requested SensiQ biomolecular interaction system is a versatile fluidics-based, dual channel SPR sensing system that can be used to determine equilibrium receptor-ligand binding affinities and kinetic constants for the association of either biological macromolecules or intact cells with displayed molecular receptors. Beyond its important impact on our specific research programs, acquisition of this SPR system will enhance interdisciplinary research and training efforts in biological physics, molecular biology, the Centre for Advanced Research in Environmental Genomics (CAREG) and the nascent Centre for Biomaterials Research, and will provide an important resource for the training of HQP in the general areas of molecular biophysics, cell biology, and the quantitative biomedical sciences.