Gas chromatography-mass spectrometer for hormone quantitation

我的NSERC资助的研究涉及了解植物激素如何调节参与植物生长和发育协调的生理和生化过程。为了研究激素之间的相互作用，并提出关于植物激素(特别是生长素、赤霉素和脱落酸)如何相互作用以协调植物不同组织之间的生长过程以及对不断变化的环境条件做出反应的工作假说，明确鉴定和定量测定内源激素以及激素前体和分解代谢产物是绝对必要的。质谱学是唯一能提供有效开展这项研究所需的绝对化合物鉴定和可重复性定量分析的技术。气相色谱与质谱仪(GC-MS)的结合是定量分析植物激素及其前体和分解代谢物的理想选择，这些激素及其前体和分解代谢物是在我的NSERC研究项目以及共同申请者Weselake博士和Strelkov博士的项目中研究的。

My NSERC funded research involves understanding how plant hormones regulate physiological and biochemical processes involved in the coordination of plant growth and development. In order to study hormonal interactions and develop working hypotheses on how plant hormones (specifically auxins, gibberellins, and abscisic acid) interact to coordinate plant growth processes between various plant tissues, and to respond to changing environmental conditions, definitive identification and quantitation of endogenous hormones, as well as hormone precursors and catabolic products are absolutely necessary. Mass spectrometry is the only technique that provides absolute compound identification and reproducible quantitative analysis needed to undertake this research effectively. The combination of gas chromatography coupled with a mass spectrometer (GC-MS) is ideal for quantitative analysis of plant hormones, and their precursors and catabolites studied in my NSERC research program and those of interest in the programs of the co-applicants, Drs. Weselake and Strelkov.