High-resolution mass spectrometer with gas chromatography

带气相色谱的高分辨率质谱仪

• 批准号: 501309152
• 金额: --
• 依托单位: Johannes Gutenberg-Universität Mainz
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/501309152?language=en
• 关键词: resolution; mass; spectrometer; gas; chromatography

High-resolution mass spectrometry is currently the most powerful and flexible technique in organic trace analysis, which is used for a wide variety of applications. This applies both to the quantitative determination of known target analytes at very low concentrations with high selectivity (target analysis), and especially when, as in the case of non-target analysis, mass spectrometry is to be used to obtain as much information as possible about the sample, for example in the case of individual samples that are difficult to access, but where a maximum of chemical information is nevertheless to be extracted. However, high-resolution mass spectrometry is not only helpful for trace analysis, but also when organic-preparation-oriented working groups aim at structure elucidation or structure detection. In many cases, the mass spectrometric high resolution enables an unambiguous elucidation of the elemental composition and also provides information for chemical structure elucidation via the interpretation of the mass spectrum. This is also true for the combination of GC and MS, as will be acquired here, with the goal of working with full-scan acquisition with very high mass resolution and mass accuracy while detecting very low concentrations. Compared to LC-MS, GC-MS captures a different range of volatility and polarity, tends to be the more robust technique, with reproducible retention times, universally applicable retention indices, and when using electron ionization, much lower compound-dependent ionization efficiencies. But softer ionization techniques, such as chemical ionization, also help in the identification of unknown compounds. The present equipment procurement is especially needed for the detection and identification of smaller organic compounds, since the existing high-resolution mass spectrometric systems are coupled with LC or are only available to a very limited extent. For this reason, the requested instrument is indispensable for the working group of the main applicant, but also for the other named working groups of the Department of Chemistry.

高分辨率质谱是目前有机痕量分析中最强大和最灵活的技术，用于各种各样的应用。这适用于以高选择性定量测定极低浓度的已知目标分析物（目标分析），特别是当如在非目标分析的情况下，质谱法用于获得尽可能多的关于样品的信息时，例如在难以访问的单个样品的情况下，但是其中仍然要提取最多的化学信息。然而，高分辨率质谱法不仅有助于痕量分析，而且当面向有机制备的工作组致力于结构阐明或结构检测时也是如此。在许多情况下，质谱的高分辨率使得能够明确地阐明元素组成，并且还通过质谱的解释提供用于化学结构阐明的信息。这对于GC和MS的组合也是如此，如将在这里获得的，其目标是在检测非常低的浓度的同时以非常高的质量分辨率和质量准确度进行全扫描采集。与LC-MS相比，GC-MS捕获不同范围的挥发性和极性，往往是更稳健的技术，具有可重现的保留时间，普遍适用的保留指数，并且当使用电子电离时，化合物依赖性电离效率低得多。但较软的电离技术，如化学电离，也有助于识别未知化合物。目前采购的设备特别需要用于检测和鉴定较小的有机化合物，因为现有的高分辨率质谱系统与LC相结合，或者只能在非常有限的程度上使用。因此，所需的仪器对于主申请人的工作组以及化学系的其他指定工作组都是必不可少的。