Developing low-intensity pulsed ultrasound device for microorganism growth

开发用于微生物生长的低强度脉冲超声装置

• 批准号: 365241-2008
• 负责人: Chen, Jie
• 金额: $ 10.82万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=434508
• 关键词: Developing; low; intensity; pulsed; ultrasound

Health care spending continues to grow faster than our economy and becomes a major financial burden to the society (Provincial and territorial government health spending surpassed $100 billion in 2007). Drugs have consumed the second-largest share of health dollars (second after Hospitals) since 1997. The fermentation process is commonly used in the pharmaceutical industry to grow microorganisms and produce drugs, like antibiotics. In this strategic research, the applicants propose to collaborate with researchers at Apotex Fermentation Inc. and Alberta Research Council to design low-intensity pulsed ultrasound (LIPUS) devices for microorganism growth. LIPUS is chosen because a form of mechanical energy generated by the LIPUS device can pass through into microorganism as acoustic pressure waves that can stimulate microorganism growth. Our piezoelectric transducers are biocompatible and are easily modified for fermentation use. The proposed research includes: (i) developing a novel LIPUS device that can stimulate microbial growth and enhance gene and protein expressions to increase fermentation-based drug yield; (ii) demonstrating that the proof-of-concept designs can be used in the fermentation processes to produce predictable microbial growth; (iii) extending this new technology for industrial applications. Our design is innovative because no research group worldwide has proposed to use LIPUS for enhancing microorganism growth.

保健支出的增长速度继续超过我国经济的增长速度，成为社会的一个主要财政负担（2007年，省和地区政府的保健支出超过1 000亿加元）。自1997年以来，药品消耗了卫生资金的第二大份额（仅次于医院）。发酵过程通常用于制药工业中培养微生物和生产药物，如抗生素。在这项战略研究中，申请人提议与Apotex Fermentation Inc.的研究人员合作。和阿尔伯塔研究理事会设计低强度脉冲超声波（LIPUS）装置的微生物生长。选择LIPUS是因为由LIPUS装置产生的机械能的形式可以作为可以刺激微生物生长的声压波通过进入微生物。我们的压电传感器具有生物相容性，易于修改以用于发酵。拟议的研究包括：（i）开发一种新型LIPUS装置，可以刺激微生物生长并增强基因和蛋白质表达，以增加基于发酵的药物产量;（ii）证明概念验证设计可以用于发酵过程，以产生可预测的微生物生长;（iii）将这种新技术扩展到工业应用。我们的设计是创新的，因为世界上没有研究小组提出使用LIPUS来促进微生物生长。