Scintillation counter

闪烁计数器

• 批准号: 390407-2010
• 负责人: DeCatanzaro, Denys
• 金额: $ 3.96万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443187
• 关键词: Scintillation; counter

This application is for a new scintillation counter, which is a machine that measures radioactivity in small samples of biological tissues or other materials. We need this to replace an aging counter that we purchased in 1984, which now has very reduced sensitivity and multiple mechanical problems. We are using this to trace tritiated (3H-) steroids between individuals. We are examining the possibility that steroid hormones such as estradiol, the most powerful estrogen, not only act within individuals but also pass between individuals. We have found that estradiol and other small steroids are excreted by adult male mice and other mammals. This is associated with two well-known pheromonal effects: 1) the loss of pregnancy in females caused by contact with unfamiliar adult males, and 2) early puberty in juvenile females exposed to unfamiliar adult males. These effects can both be mimicked by injecting females with estradiol. We are conducting experiments with mice where we trace 3H-estradiol from males' systems into their urine and other excretions, then measure this 3H-estradiol in the uterus and brain of nearby females. This is very meaningful given evidence that small elevations of estradiol in the uterus can disturb early pregnancy. Furthermore, estradiol promotes puberty, as growth of the uterus is naturally driven by estradiol and female sexual behaviour is naturally stimulated by the actions of estradiol in the hypothalamus of the brain. Accordingly, this new scintillation counter is critical for our research identifying the chemical nature of important reproductive pheromones. A new scintillation counter will also help this laboratory's ability to measure steroids in blood, urine, and other media, as it will allow us to use sensitive radioimmunoassay procedures. This counter is critical for the continuation of the research of students already in the laboratory, and it will help future students to conduct innovative research in pheromone dynamics and endocrine disruption. It will help to ensure that this laboratory remains competitive, making substantial contributions to basic reproductive science and reproductive toxicology.

这是一种新的闪烁计数器的应用，它是一种测量生物组织或其他材料小样本放射性的机器。我们需要这个来替换1984年购买的老化计数器，现在老化计数器的灵敏度降低了，而且出现了多种机械问题。我们用它来追踪个体之间的氚化（3H-）类固醇。我们正在研究类固醇激素，如雌二醇，最强大的雌激素，不仅在个体内起作用，而且在个体之间传递的可能性。我们发现雌二醇和其他小类固醇是由成年雄性老鼠和其他哺乳动物排泄的。这与两种众所周知的信息素效应有关：1)与不熟悉的成年雄性接触导致雌性流产；2)与不熟悉的成年雄性接触导致雌性早熟。这些效果都可以通过给雌性注射雌二醇来模拟。我们正在用老鼠做实验，我们在它们的尿液和其他排泄物中追踪雄性系统中的3h -雌二醇，然后测量附近雌性子宫和大脑中的3h -雌二醇。这是非常有意义的，因为有证据表明，子宫内雌二醇的轻微升高会扰乱早期妊娠。此外，雌二醇促进青春期，因为子宫的生长是由雌二醇自然驱动的，女性的性行为是由雌二醇在大脑下丘脑中的作用自然刺激的。因此，这种新的闪烁计数器对于我们研究识别重要生殖信息素的化学性质至关重要。一个新的闪烁计数器也将有助于本实验室测量血液，尿液和其他介质中的类固醇，因为它将允许我们使用敏感的放射免疫测定程序。这个计数器对于学生已经在实验室进行的研究的继续是至关重要的，它将帮助未来的学生在信息素动力学和内分泌干扰方面进行创新研究。它将有助于确保该实验室保持竞争力，为基础生殖科学和生殖毒理学作出重大贡献。