Thermal cycler for the detection and manipulation of ion channel genes

用于检测和操作离子通道基因的热循环仪

• 批准号: 390623-2010
• 负责人: Campanucci, Veronica
• 金额: $ 0.75万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443243
• 关键词: Thermal; cycler; detection; manipulation; ion

In this equipment grant I am requesting funds for the purchase of a thermal cycler to detect and manipulate genes for ion channels from sympathetic neurons. This equipment is required to assist in the site-directed mutagenesis, amplification, detection and deletion of genes for ion channel subunits. Current research in my laboratory focuses on understanding neuronal function, which is directly determined by the activity and function of ion channels. I will use the requested equipment to investigate how ion channels work and how they are modulated during stress conditions. Conditions such as those generated by oxidative stress can alter the structure of ion channel proteins, and therefore, affect their function. This equipment will assist our research on how serotoninergic type 3 receptor (5HT3R) function during physiological conditions and during oxidative stress. In the future, my research program will extend to other ion channels in diverse cellular models and expression systems, making the acquirement of this equipment highly important for my research program for many years to come. The equipment can (and will) also be used by colleagues both inside and outside of my department. Furthermore, this particular system has been improved with state-of-the-art components that make it extremely accurate and reliable for DNA work. This equipment will assist graduate students and trainees at the Department of Physiology and at the Neural Systems & Plasticity Research Group, being of great advantage to the training of highly qualified personnel.

在这个设备补助金中，我申请购买热循环仪的资金，以检测和操纵交感神经元离子通道的基因。需要该设备来协助离子通道亚基基因的定点诱变、扩增、检测和缺失。我实验室目前的研究重点是了解神经元的功能，这直接取决于离子通道的活性和功能。我将使用所要求的设备来研究离子通道如何工作以及它们在应激条件下如何被调节。氧化应激等条件可以改变离子通道蛋白的结构，从而影响其功能。该设备将有助于我们研究在生理条件下和氧化应激期间，肾上腺素能3型受体（5HT3R）如何发挥作用。在未来，我的研究计划将扩展到不同细胞模型和表达系统中的其他离子通道，这使得该设备的获得对我未来多年的研究计划非常重要。我的部门内外的同事也可以（也将）使用这些设备。此外，这个特殊的系统已经改进了最先进的组件，使其非常准确和可靠的DNA工作。该设备将帮助生理学系和神经系统与可塑性研究小组的研究生和实习生，对培养高素质人才具有很大的优势。