Thermal cycler for the detection and manipulation of ion channel genes

在这项设备拨款中，我申请了一笔资金，用于购买一台热循环仪，以检测和操纵交感神经元离子通道的基因。这种设备是协助离子通道亚单位基因的定点突变、扩增、检测和删除所必需的。我的实验室目前的研究重点是了解神经元的功能，这直接由离子通道的活动和功能决定。我将使用所需的设备来研究离子通道是如何工作的，以及它们在应激条件下是如何调节的。氧化应激等条件会改变离子通道蛋白的结构，从而影响其功能。这台设备将帮助我们研究5-羟色胺能3型受体(5HT3R)在生理条件和氧化应激过程中如何发挥作用。在未来，我的研究计划将扩展到不同细胞模型和表达系统中的其他离子通道，这使得获得这种设备对我未来多年的研究计划非常重要。我的部门内外的同事也可以(也将会)使用这些设备。此外，这个特殊的系统已经得到了改进，使用了最先进的组件，使其非常准确和可靠，用于DNA工作。该设备将帮助生理学系和神经系统与可塑性研究组的研究生和实习生，对培养高素质人才具有极大的优势。

In this equipment grant I am requesting funds for the purchase of a thermal cycler to detect and manipulate genes for ion channels from sympathetic neurons. This equipment is required to assist in the site-directed mutagenesis, amplification, detection and deletion of genes for ion channel subunits. Current research in my laboratory focuses on understanding neuronal function, which is directly determined by the activity and function of ion channels. I will use the requested equipment to investigate how ion channels work and how they are modulated during stress conditions. Conditions such as those generated by oxidative stress can alter the structure of ion channel proteins, and therefore, affect their function. This equipment will assist our research on how serotoninergic type 3 receptor (5HT3R) function during physiological conditions and during oxidative stress. In the future, my research program will extend to other ion channels in diverse cellular models and expression systems, making the acquirement of this equipment highly important for my research program for many years to come. The equipment can (and will) also be used by colleagues both inside and outside of my department. Furthermore, this particular system has been improved with state-of-the-art components that make it extremely accurate and reliable for DNA work. This equipment will assist graduate students and trainees at the Department of Physiology and at the Neural Systems & Plasticity Research Group, being of great advantage to the training of highly qualified personnel.