Thermal cycler for the detection and manipulation of ion channel genes

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In this equipment grant I am requesting funds for the purchase of a thermal cycler to detect and manipulate genes for ion channels from sympathetic neurons. This equipment is required to assist in the site-directed mutagenesis, amplification, detection and deletion of genes for ion channel subunits. Current research in my laboratory focuses on understanding neuronal function, which is directly determined by the activity and function of ion channels. I will use the requested equipment to investigate how ion channels work and how they are modulated during stress conditions. Conditions such as those generated by oxidative stress can alter the structure of ion channel proteins, and therefore, affect their function. This equipment will assist our research on how serotoninergic type 3 receptor (5HT3R) function during physiological conditions and during oxidative stress. In the future, my research program will extend to other ion channels in diverse cellular models and expression systems, making the acquirement of this equipment highly important for my research program for many years to come. The equipment can (and will) also be used by colleagues both inside and outside of my department. Furthermore, this particular system has been improved with state-of-the-art components that make it extremely accurate and reliable for DNA work. This equipment will assist graduate students and trainees at the Department of Physiology and at the Neural Systems & Plasticity Research Group, being of great advantage to the training of highly qualified personnel.