Polymer synthesis of biomaterials for restoring hard and soft tissue function in oral-facila structures

这一发现的潜在假设是，口腔液体的酯酶生物降解模型系统可以定义聚合物生物材料结构，从而能够设计生物稳定的牙齿修复材料，以及具有肽功能的生物可降解支架，用于再生功能性粘膜组织。这个发现基金将有三个主要主题。PI先前的工作已经确定了口腔唾液液中最有效的酶复合物，这些酶复合物负责牙齿修复中使用的传统聚合物复合材料的生化分解。更新发现基金的目标是使用上述酶复合物的模型酶系统来系统地识别新的单体结构，这些结构可用于构思高度生物稳定和抗菌的牙齿修复材料。在其他工作中，研究者的实验室已经证明，通过从复合聚合物中释放低ppm至ppb水平的降解产物，可以在分子水平上调节细菌的生物功能。因此，这项更新发现基金将探索新单体的合成，以产生可以释放抗菌药物的复合材料，同时履行其作为牙齿填充物的功能。最后一个研究主题将探讨支持粘膜软组织细胞的微孔支架结构的制备。这项工作将强调三个主要的设计参数，这些参数被认为是口腔软组织再生的最终目标所必需的，包括单体转化、生物降解率和三维支架内的细胞增殖。这些材料最终可能用于修复因年龄、遗传和环境相关疾病而受损的组织。这项资助的研究结果将为单体化学结构对生物材料的物理、生化和生物学结果的影响提供更深入的机制理解，旨在用于对加拿大人产生生理、心理/社会和经济影响的健康相关领域（即口腔健康科学）。这些聚合物也可能在农业和环境管理系统的生物输送概念中具有潜在的用途。

The underlying hypothesis of this discovery grant is that model esterase biodegradation systems for oral fluids can define polymeric biomaterial structures that will enable the design of biostable tooth restorative materials, as well as biodegradable scaffolds with peptide function for regenerating functional mucosal tissues. There will be 3 main themes to this discovery grant. Previous work by the PI has identified the most potent enzyme complexes in oral salivary fluids which are responsible for the biochemical breakdown of traditional polymeric composite materials used in tooth restorations. It is an objective of the renewal discovery grant to use model enzyme systems for the above enzyme complexes to methodically identify novel monomeric structures that could be used to conceive highly biostable and antimicrobial tooth restorative materials. In other work the investigator's lab has demonstrated that it is possible to modulate the biological function of bacteria at the molecular level with the release of low ppm to ppb levels of degradation products from composite polymers. Therefore, this renewal discovery grant will explore the synthesis of new monomers to generate composite materials which could release antimicrobial agents while fulfilling their functional role as a tooth filling. The last research theme will explore the fabrication of micro-porous scaffold structures that could support mucosal soft tissue cells.This work will emphasis 3 principle design parameters, believed to be essential in the ultimate goal of regenerating soft tissue in the oral cavity, including monomer conversion, biodegradation rates and cell proliferation within the 3-dimensional scaffolds. These materials may ultimately be used to restore tissue damaged by age, genetic and environmental related illnesses. The findings from this grant will provide a greater mechanistic understanding for the influence of monomer chemical structure on the physical, biochemical and biological outcomes of biomaterials, intended for a health related area (i.e. the oral-health sciences) that has physical, psychological/societal, and economic impact on Canadians. These polymers may also have potential uses in biological delivery concepts for agriculture and environmental management systems.