Novel Mass Spectrometric Approaches to the Study of Protein-Protein Interactions

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The proposed research will develop novel mass spectrometric approaches for the study of biologically important protein assemblies, to derive structural and functional information for systems otherwise not amenable to traditional analytical methods. This will be achieved by integrating and extending the capabilities of negative ion mass spectrometry, ion mobility and chemical cross-linking strategies. The significance of the novel methodologies will be demonstrated by application to selected challenging biological systems relevant to human health, including 1) the study of protein misfolding and aggregation pathways, and 2) structural analysis of peptide-protein complex formation.