Bilayer clamp

双层夹

• 批准号: 406190-2011
• 负责人: Fyles, Thomas
• 金额: $ 2.49万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472406
• 关键词: Bilayer; clamp

This proposal seeks urgent funding to replace a bilayer clamp system that is essential for the continued progress of Discovery Grant funded research. A bilayer clamp system is a specialized two-electrode potentiostat system which applies potential (mV) and measures current (pA) across a supported bilayer membrane. The membrane without a functioning channel is an insulator, so an applied potential results in no current. When a channel opens, ions flow and the current is detected as a sharp jump; when the channel closes, the current abruptly drops to zero. The analysis of this on-off behavior can be related to the diameter and stability of the ion channel at the single-molecule level.

这项提案寻求紧急资金，以取代双层夹系统，这是必不可少的继续进展的发现补助金资助的研究。 双层夹系统是一种专门的双电极恒电位仪系统，其施加电势（mV）并测量跨支撑的双层膜的电流（pA）。 没有功能通道的膜是绝缘体，因此施加的电势不会产生电流。 当通道打开时，离子流动，电流被检测为急剧跳跃;当通道关闭时，电流突然下降到零。 这种开关行为的分析可以在单分子水平上与离子通道的直径和稳定性相关。