High performance imaging and image analysis unit

高性能成像和图像分析单元

• 批准号: 406641-2011
• 负责人: Chartrand, Pascal
• 金额: $ 3.64万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472526
• 关键词: performance; imaging; image; analysis; unit

Researchers in our department rely on a single inverted epifluorescence microscope for fixed and live cell imaging of mammalian cells. This microscope is heavily used and is currently equipped with an interline CCD camera, purchased in 2002. As our needs for fluorescence microscopy has moved toward the imaging of low light, single particles in individual cells, this camera is no longer appropriate. The requested sCMOS camera has a larger chip with small pixels and lower read noise compared to the interline CCD camera, which allows the acquisition of larger field of view with high resolution, low background and wide dynamic range. This camera will be combined to the epifluorescence microscope, which is already equipped for microinjection and environmental control for imaging live mammalian cells. As we will be able to acquire images of better quality at higher sensitivity with the sCMOS camera, we also request the high performance analytical imaging software Imaris. This software has the capability to extract and analyze quantitative information from images or movies, perform particle tracking in 3D, volume rendering and colocalization studies. Both high performance sCMOS camera and Imaris analytical image processing software are highly complementary and will generate considerable synergy in our research programs.

我们系的研究人员依靠单台倒置荧光显微镜对哺乳动物细胞进行固定细胞和活细胞成像。这台显微镜被大量使用，目前配备了一个线间CCD相机，购买于2002年。由于我们对荧光显微镜的需求已经转向弱光成像，单个细胞中的单个颗粒，这种相机不再合适。与行间CCD相机相比，所要求的sCMOS相机具有更大的芯片，像素更小，读取噪声更低，可以获得高分辨率，低背景和宽动态范围的大视场。这台摄像机将与荧光显微镜相结合，后者已经装备用于显微注射和环境控制，以成像活体哺乳动物细胞。由于我们将能够以更高的灵敏度获得更高质量的图像，我们还要求高性能分析成像软件Imaris。该软件具有从图像或电影中提取和分析定量信息的能力，在3D中执行粒子跟踪，体渲染和共定位研究。高性能sCMOS相机和Imaris分析图像处理软件是高度互补的，将在我们的研究项目中产生相当大的协同作用。