A platform for simultaneous optical and electrical single-molecule detection through nanopore arrays

通过纳米孔阵列同时进行光学和电学单分子检测的平台

• 批准号: 407831-2011
• 负责人: TabardCossa, Vincent
• 金额: $ 10.03万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2010
• 资助国家: 加拿大
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=472729
• 关键词: platform; simultaneous; optical; electrical; single

The aim of this proposal is to build a platform for simultaneous optical and electrical single-molecule detection through nanopore arrays. In recent years, the field of biological physics has seen many of its most exciting developments owing to the emergence of tools to probe individual molecular processes. Further advancement will require the implementation of new tools to circumvent the limitations imposed by current technologies. Among these, tools that combine force spectroscopy methods with other single-molecule techniques, in particular single molecule fluorescence detection, represent an important development when addressing increasingly complex biological questions. Another major advancement hinges on the increase in parallelism and throughput of single-molecule force spectroscopy measurements. The requested equipment will be capable of addressing these key challenges. This unique and versatile system will enable a broad range of single-molecule measurements currently not possible in Canada, such as parallel force spectroscopy on an array of solid-state nanopores for rapid characterization of intermolecular interactions.

这一提议的目的是通过纳米孔阵列建立一个同时进行光学和电学单分子检测的平台。近年来，由于探测单个分子过程的工具的出现，生物物理领域出现了许多最令人兴奋的发展。要取得进一步的进展，就需要采用新的工具，以绕过现有技术造成的限制。其中，将力光谱方法与其他单分子技术相结合的工具，特别是单分子荧光检测，在解决日益复杂的生物学问题时代表着一项重要的发展。另一项重大进展取决于单分子力谱测量的并行度和吞吐量的增加。所要求的设备将能够应对这些关键挑战。这一独特而多功能的系统将使目前在加拿大无法实现的广泛的单分子测量成为可能，例如在一系列固态纳米孔上进行平行力光谱，以快速表征分子间相互作用。