Plant and soil analytical sample preparation system

植物和土壤分析样品制备系统

• 批准号: RTI-2017-00395
• 负责人: Cahill, James
• 金额: $ 3.08万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616662
• 关键词: Plant; soil; analytical; sample; preparation

I am requesting funds to purchase an integrated system allowing for the accurate and rapid processing of biological samples (plant tissue and soils) in support of my ongoing and future research in plant ecology. Broadly, my research program focuses on obtaining a mechanistic understandings of plant-plant interactions, plant-soil interactions, plant-chemical ecology, community assembly, plant foraging behaviour, and the impacts of disturbance and change (e.g. invasion, warming, herbivory, etc) on biodiversity and ecosystem function. Combined these research areas are critical to helping Canada mitigate the negative consequences of many human-induced changes to natural systems. Further, this information will better inform our basic understanding of natural systems and plant biology, with implications both for an economy heavily dependent upon natural resources and for our ability to maintain Canada’s biodiversity into the future. The proposed system has two phases (1) weight determination, and (2) tissue disruption. Sample processing is fundamental to the research conducted in my lab, and at least one of the two stages is performed on approximately 10,000 samples per year. These funds will be used to purchase two analytical balances (including one highly precise microbalance), a bead mill to grind biological samples, and control software and hardware. The existing balances in my lab have outlived their useful lives, and are no longer able to be calibrated. Failure to have reliable balances introduces error at the heart of all scientific inquires in my research group. Consequently, the balances are in urgent need of replacement. My research group does not currently have a bead mill for high-throughput tissue disruption, and we have instead borrowed time on those in the labs of colleagues. As my research program has increased its dependence on methods drawn from molecular biology and chemistry, I have also increased the number of samples generated each year requiring tissue disruption allow proper chemical reactions. At this point, the number of samples my research group processes annually is too great to be accommodated by equipment in other labs, without causing negative impacts on the research activities of others. Thus, it is critical this equipment be purchased so that my lab members can have unfettered access, facilitating research discovery. Though all of this equipment will be heavily used by my own lab members, I will also invite colleagues and students to use the equipment when need arises. I have benefited greatly from the generosity of colleagues, and am more than happy to offer such generosity in return.

我正在申请资金，以购买一个集成系统，允许准确和快速地处理生物样本(植物组织和土壤)，以支持我目前和未来在植物生态学方面的研究。总的来说，我的研究计划侧重于从机理上了解植物-植物相互作用、植物-土壤相互作用、植物-化学生态、群落组装、植物觅食行为，以及干扰和变化(如入侵、变暖、食草性等)对生物多样性和生态系统功能的影响。总而言之，这些研究领域对于帮助加拿大减轻许多人类引起的自然系统变化的负面后果至关重要。此外，这些信息将更好地为我们提供对自然系统和植物生物学的基本理解，对严重依赖自然资源的经济和我们在未来维持加拿大生物多样性的能力都有影响。所提出的系统包括两个阶段(1)权重确定和(2)组织破坏。样品处理是我实验室进行的研究的基础，每年至少要对大约10,000个样品进行两个阶段中的一个。这些资金将用于购买两台分析天平(包括一台高精度微量天平)、一台研磨生物样本的珠磨机以及控制软件和硬件。我实验室中现有的天平已经超过了它们的使用寿命，不能再进行校准。没有可靠的平衡会导致错误，这是我所在研究小组所有科学调查的核心。因此，这些余额迫切需要更换。我的研究小组目前没有用于高通量组织破坏的珠磨机，相反，我们借用了同事实验室的时间。随着我的研究项目越来越依赖从分子生物学和化学中提取的方法，我也增加了每年产生的样本数量，需要组织破坏才能进行适当的化学反应。在这一点上，我的研究小组每年处理的样本数量太大，其他实验室的设备无法容纳，而不会对其他实验室的研究活动造成负面影响。因此，购买这种设备是至关重要的，这样我的实验室成员就可以不受限制地访问，促进研究发现。虽然所有这些设备都会被我自己的实验室成员大量使用，但如果有需要，我也会邀请同事和学生使用这些设备。同事们的慷慨使我受益匪浅，我也非常乐意提供这样的慷慨作为回报。