Imaging System for Cellular and Molecular Toxicology

细胞和分子毒理学成像系统

• 批准号: 422745-2012
• 负责人: Cyr, Daniel
• 金额: $ 4.32万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495844
• 关键词: Imaging; System; Cellular; Molecular; Toxicology

The role of intracellular signalling pathways in cell-cell interactions in toxicology has led to significant new findings regarding the mechanisms by which environmental toxicants can cause deleterious effects on various physiological systems. The current application is to update our image analysis system which is almost 14 years old and has become unserviceable and unreliable for the long-term. We propose to acquire a versatile image analysis system that can be used for measuring fluorescence and chemiluminescence in addition to UV and epi-white light. Fluorescent imaging and chemiluminescence using a sensitive CCD camera offers many advantages in terms of sensitivity and linearity over several orders of magnitude, as well as lower costs, as compared to streptavidin and chemiluminescence or radioactivity with x-ray films. More importantly, it will provide a highly sensitive and reliable imaging system for analyzing western blots, in-cell westerns, electrophoretic mobility shift assays, DNA laddering for apoptosis and documenting Coomassie blue stained gels.

细胞内信号通路在毒理学中细胞-细胞相互作用中的作用导致了关于环境毒物对各种生理系统造成有害影响的机制的重要新发现。目前的应用程序是更新我们的图像分析系统，该系统已使用近14年，长期以来已无法使用和不可靠。我们建议获得一个多功能的图像分析系统，可用于测量荧光和化学发光，除了紫外和epi-white光。 与链霉亲和素和化学发光或X射线胶片的放射性相比，使用灵敏的CCD照相机的荧光成像和化学发光在灵敏度和线性度方面提供了许多优点，并且成本较低。 更重要的是，它将提供一个高灵敏度和可靠的成像系统，用于分析蛋白质印迹，细胞内蛋白质印迹，电泳迁移率变动分析，DNA梯状凋亡和记录考马斯蓝染色凝胶。