Imaging System for Cellular and Molecular Toxicology

在毒理学中，细胞内信号通路在细胞-细胞相互作用中的作用导致了关于环境毒物对各种生理系统造成有害影响的机制的重大新发现。目前的应用是更新我们的图像分析系统，它已经使用了近14年，已经变得无法使用，长期不可靠。我们建议获得一个通用的图像分析系统，除了紫外光和表白光外，还可以测量荧光和化学发光。与链霉亲和素和化学发光相比，使用灵敏的CCD相机的荧光成像和化学发光在几个数量级的灵敏度和线性度方面具有许多优势，而且成本更低。更重要的是，它将提供一个高度敏感和可靠的成像系统，用于分析蛋白质印迹、细胞内的蛋白质、电泳迁移率变化分析、DNA梯形分析细胞凋亡和记录考马斯西蓝染色的凝胶。

The role of intracellular signalling pathways in cell-cell interactions in toxicology has led to significant new findings regarding the mechanisms by which environmental toxicants can cause deleterious effects on various physiological systems. The current application is to update our image analysis system which is almost 14 years old and has become unserviceable and unreliable for the long-term. We propose to acquire a versatile image analysis system that can be used for measuring fluorescence and chemiluminescence in addition to UV and epi-white light. Fluorescent imaging and chemiluminescence using a sensitive CCD camera offers many advantages in terms of sensitivity and linearity over several orders of magnitude, as well as lower costs, as compared to streptavidin and chemiluminescence or radioactivity with x-ray films. More importantly, it will provide a highly sensitive and reliable imaging system for analyzing western blots, in-cell westerns, electrophoretic mobility shift assays, DNA laddering for apoptosis and documenting Coomassie blue stained gels.