High performance liquid chromatography electrospray tandem mass spectrometry for quantitative determination of biomarkers, drugs and metabolites in biological matrices

高效液相色谱电喷雾串联质谱法用于定量测定生物基质中的生物标志物、药物和代谢物

• 批准号: 439748-2013
• 负责人: Beaudry, Francis
• 金额: $ 10.37万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=505651
• 关键词: performance; liquid; chromatography; electrospray; tandem

This proposition includes the acquisition of a QTRAP LC-MS/MS System. This instrument is a hybrid mass spectrometer that combines many conventional ion trap functionalities but deliver triple quadrupole quantitative performance. This instrument is crucial since we retired an 19 years old SCIEX API 3+ instrument in June 2011. Thus, the acquisition of the QTRAP LC-MS/MS is critical for the success of our research program supported by an NSERC discovery grant entitled:"Discovery of neuroproteomic determinants of pain transmission using differential and systematic proteome exploration strategies" received in March 2010 for a period of 5 years. The QTRAP LC-MS/MS instrument offers unmatched qualitative performance for proteomic, peptidomic and drug discovery, as well as the wide dynamic range required for accurate quantitation central to our research activities. Furthermore, the instrument proposed is needed to quantify protein expression using multiplexed isobaric tagging technology and specific multiple reaction monitoring (MRM) into experimental animal models of chronic and neuropathic pain. MRM techniques are the gold standard in quantitative biomarkers discovery and validation. The performance of protein and peptide quantification methods is important in biomarker research since it is used to determine protein expression, performed principal component analysis (PCA) and define valid multiparametric profiles for diagnostic and prognostic purposes. Additionally, these types of instrument are an excellent choice to perform quantification of drug and metabolites in complex biological matrices. Drug pharmacokinetic and metabolism are key elements of animal pharmacology research and an integral part of our highly qualified scientists training program in biomedical research. The acquisition of the QTRAP LC-MS/MS will significantly contribute to our effort to develop new mechanistic knowledge of pain transmission, identify specific biomarkers and foster the basis for the development of innovative pain therapies. This new LC-MS/MS will have an immediate impact into our research activities and the training of Highly Qualified Personnel (HQP).

这一提议包括收购QTRAP LC-MS/MS系统。该仪器是一种混合质谱仪，结合了许多传统的离子陷阱功能，但提供了三重四极杆的定量性能。自从2011年6月我们退役了一台有19年历史的SCIEX API 3+仪器以来，这台仪器就变得至关重要。因此，收购QTRAP LC-MS/MS对于我们的研究计划的成功至关重要，该研究计划由NSERC发现基金支持，该基金于2010年3月收到为期5年的“使用差异和系统蛋白质组探索策略发现疼痛传递的神经蛋白质组决定因素”。QTRAP LC-MS/MS仪器为蛋白质组、多肽和药物发现提供了无与伦比的定性性能，以及我们研究活动所需的准确定量所需的广泛动态范围。此外，所提出的仪器需要使用多路等压标记技术和特异性多反应监测(MRM)来量化慢性和神经病理性疼痛的实验动物模型中的蛋白质表达。MRM技术是定量生物标记物发现和验证的黄金标准。蛋白质和多肽定量方法的性能在生物标记物研究中非常重要，因为它被用来确定蛋白质的表达，执行主成分分析(PCA)，并为诊断和预后目的定义有效的多参数曲线。此外，这些类型的仪器是对复杂生物基质中的药物和代谢物进行定量的极佳选择。药物药代动力学和新陈代谢是动物药理学研究的关键要素，也是我们在生物医学研究方面的高资质科学家培训计划的组成部分。收购QTRAP LC-MS/MS将大大有助于我们开发新的疼痛传递机制知识，识别特定的生物标记物，并为创新疼痛疗法的开发奠定基础。这一新的LC-MS/MS将对我们的研究活动和高素质人员(HQP)的培训产生直接影响。