Neural control of respiration in lampreys

七鳃鳗呼吸的神经控制

• 批准号: 217435-2007
• 负责人: Dubuc, Réjean
• 金额: $ 3.11万
• 依托单位: Université du Québec à Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=514202
• 关键词: Neural; control; respiration; lampreys

Respiration is a vital motor behaviour that begins at birth and is maintained throughout life. Pathological changes in breathing can have dire consequences and it is thus crucial to understand the neural mechanisms generating respiration. To do this, both the synaptic connectivity of the entire network as well as the membrane properties of the comprising generator neurons must be identified. The complexity of the mammalian Central Nervous System (CNS) renders this task difficult and we believe that the simpler CNS of lampreys could help provide a detailed understanding. In lampreys, the neural circuitry generating respiration stays active after isolation in vitro of their CNS, and continues to generate respiratory output very similar to that observed in intact animals. We propose to characterize the neural circuitry both at the system (synaptic connectivity) and cellular (intrinsic properties of neurons) levels in the CNS of lampreys. The relative contribution of synaptic and intrinsic properties in respiration will also be established. Finally, we will examine the modulation of respiration by other motor circuits in the isolated CNS. The onset of movements is accompanied by increases in the respiratory drive and previous work from our lab has revealed that the changes are pre-programmed in the CNS. We propose to identify the neural circuitry responsible for these changes. We believe that the information gained in this lower vertebrate CNS will be useful to provide testable hypotheses on the neural organization responsible for respiration in higher vertebrates, including humans.

呼吸是一种重要的运动行为，从出生开始，并在整个生命中保持。呼吸的病理变化可能会产生可怕的后果，因此了解产生呼吸的神经机制至关重要。要做到这一点，整个网络的突触连接性以及组成生成器神经元的膜特性都必须被识别。哺乳动物中枢神经系统（CNS）的复杂性使得这项任务变得困难，我们相信七鳃鳗的简单CNS可以帮助提供详细的了解。在七鳃鳗中，产生呼吸的神经回路在其CNS体外分离后保持活跃，并继续产生与在完整动物中观察到的非常相似的呼吸输出。我们建议在系统（突触连接）和细胞（神经元的内在属性）水平上表征七鳃鳗中枢神经系统的神经回路。突触和内在属性在呼吸中的相对贡献也将被建立。最后，我们将研究呼吸的调制由其他运动电路在孤立的中枢神经系统。运动的开始伴随着呼吸驱动的增加，我们实验室以前的工作表明，这些变化是在CNS中预先编程的。我们建议确定负责这些变化的神经回路。我们相信，在这较低的脊椎动物中枢神经系统获得的信息将是有用的，提供可验证的假设，负责呼吸的神经组织在高等脊椎动物，包括人类。