Time-of-flight mass spectrometry of biomolecules using particle and laser induced desorption

使用粒子和激光诱导解吸对生物分子进行飞行时间质谱分析

• 批准号: 38415-2011
• 负责人: Ens, Werner
• 金额: $ 1.6万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=568815
• 关键词: Time; flight; mass; spectrometry; biomolecules

The dramatic developments in gene technology, and the completion of genome sequences for a variety of organisms, including humans, provide unprecedented opportunities for rapid and sensitive determination of the identity and function of proteins. In this field of proteomics, mass spectrometry plays a pivotal role, due to similarly dramatic developments in this field, many of them in physics laboratories. In the late 80s, limited accessible mass range and sensitivity were largely overcome by the invention of two new ionization techniques, matrix-assisted laser desorption/ionization (MALDI), and electrospray ionization (ESI). The 2002 Nobel prize in Chemistry was awarded in part for these developments. The broad objectives of our research are to study, develop, and apply methods of mass spectrometry for large molecules, particularly biomolecules. Orthogonal-injection MALDI TOF and the corresponding application of MS/MS using a quadrupole/TOF geometry, were first demonstrated in our laboratory, and have become standard techniques in proteomics research. The particular focus of this project is to study the physical mechanisms involved in the desorption of large molecules by laser and particle desorption and to develop improved methods for mass selected imaging of biological tissue. Many of the recent improvements in analyzing ions produced by MALDI, such as delayed extraction and orthogonal-injection depend critically on knowledge of the ejection dynamics, with and without an extraction field, and we propose to continue our work on such studies using both axial and orthogonal injection TOF instruments.

基因技术的巨大发展和包括人类在内的各种生物体基因组序列的完成为快速和灵敏地确定蛋白质的身份和功能提供了前所未有的机会。在蛋白质组学的这个领域，质谱法起着关键的作用，由于在这个领域的类似戏剧性的发展，其中许多是在物理实验室。在80年代后期，两种新的离子化技术，基质辅助激光解吸/离子化（MALDI）和电喷雾离子化（ESI）的发明在很大程度上克服了有限的可达质量范围和灵敏度。2002年诺贝尔化学奖的颁发部分是因为这些进展。 我们研究的广泛目标是研究，开发和应用大分子，特别是生物分子的质谱分析方法。在我们的实验室中首次展示了毛细管注射MALDI TOF和相应的MS/MS应用，并已成为蛋白质组学研究的标准技术。该项目的特别重点是研究通过激光和颗粒解吸对大分子进行解吸的物理机制，并开发用于生物组织质量选择成像的改进方法。许多最近的改进，如延迟提取和正交注射在分析MALDI产生的离子，关键取决于知识的喷射动力学，有和没有提取字段，我们建议继续我们的工作，使用轴向和正交注射TOF仪器进行这些研究。