Hybridized dense nano-fibrillar collagen gels as tissue models and scaffolds

杂交致密纳米纤维胶原凝胶作为组织模型和支架

• 批准号: 341235-2011
• 负责人: Nazhat, Showan
• 金额: $ 2.91万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=572207
• 关键词: Hybridized; dense; nano; fibrillar; collagen

The focus of this Discovery Program Grant is the engineering, and characterisation of the structure-property relationship of dense, tissue-like collagenous matrices developed as in vitro models, and as scaffolds for the regeneration of hard and soft tissues. Plastic compression rapidly generates biomimetic, dense collagen scaffolds by removing fluid from in vitro reconstituted, highly-hydrated nano-fibrillar collagen gels. Within minutes, three dimensional matrices are generated having collagen fibrillar densities similar to those of native extracellular matrix. Moreover, when this is applied to cellular scaffolds, cell viability is maintained and the constructs provide a supportive environment for long term cultures to investigate the effects of biochemical and biomechanical cues on tissue maturation. The method is also ideal for generating hybridized scaffolds through the incorporation of other biopolymers or bioinorganics. This program will generate novel hybrid scaffolds to investigate the role of various factors that influence cellular function in three dimensions aimed towards bone tissue engineering, vascularisation of tissues, and soft tissue regeneration. These hybrid scaffolds will be useful as in vitro tissue models and regenerative medicine research. The knowledge generated will contribute to biomaterials science and tissue engineering, and to the understanding of cellular responses to tuneable three dimensional tissue models.

这项发现计划资助的重点是作为体外模型和硬组织和软组织再生支架开发的致密组织样胶原基质的结构-性质关系的工程和表征。塑性压缩通过从体外重构的高度水合的纳米纤维状胶原凝胶中去除流体来快速产生仿生的致密胶原支架。在几分钟内，生成具有与天然细胞外基质相似的胶原纤维密度的三维基质。此外，当将其应用于细胞支架时，细胞活力得以维持，并且构建体为长期培养提供支持性环境，以研究生物化学和生物力学线索对组织成熟的影响。该方法也是通过掺入其他生物聚合物或生物无机物来产生杂交支架的理想方法。 该计划将产生新型混合支架，以研究影响细胞功能的各种因素在三维中的作用，旨在实现骨组织工程，组织血管化和软组织再生。这些混合支架将是有用的体外组织模型和再生医学研究。所产生的知识将有助于生物材料科学和组织工程，以及对可调三维组织模型的细胞反应的理解。