Hybridized dense nano-fibrillar collagen gels as tissue models and scaffolds

杂交致密纳米纤维胶原凝胶作为组织模型和支架

• 批准号: 341235-2011
• 负责人: Nazhat, Showan
• 金额: $ 2.91万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=527230
• 关键词: Hybridized; dense; nano; fibrillar; collagen

The focus of this Discovery Program Grant is the engineering, and characterisation of the structure-property relationship of dense, tissue-like collagenous matrices developed as in vitro models, and as scaffolds for the regeneration of hard and soft tissues. Plastic compression rapidly generates biomimetic, dense collagen scaffolds by removing fluid from in vitro reconstituted, highly-hydrated nano-fibrillar collagen gels. Within minutes, three dimensional matrices are generated having collagen fibrillar densities similar to those of native extracellular matrix. Moreover, when this is applied to cellular scaffolds, cell viability is maintained and the constructs provide a supportive environment for long term cultures to investigate the effects of biochemical and biomechanical cues on tissue maturation. The method is also ideal for generating hybridized scaffolds through the incorporation of other biopolymers or bioinorganics.

这项发现计划的重点是工程，以及致密的结构-性质关系的特征，组织样胶原基质作为体外模型开发，作为硬组织和软组织再生的支架。塑料压缩通过从体外重建的高水合纳米纤维胶原蛋白凝胶中去除液体，迅速生成仿生、致密的胶原蛋白支架。几分钟内，三维基质生成，其胶原纤维密度与天然细胞外基质相似。此外，当将其应用于细胞支架时，细胞活力得以维持，并且该结构为长期培养提供了支持性环境，以研究生化和生物力学线索对组织成熟的影响。该方法也是通过掺入其他生物聚合物或生物无机物来产生杂交支架的理想方法。