Engineered three dimensional tissue-equivalents

工程化三维组织等效物

• 批准号: RGPIN-2016-05363
• 负责人: Nazhat, Showan
• 金额: $ 2.77万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=709667
• 关键词: Engineered; three; dimensional; tissue; equivalents

The focus of this Discovery Grant Program is the engineering, and characterization of the structure-property relationship of dense, tissue-like collagenous matrices developed as in vitro models, and as scaffolds for the regeneration of hard and soft tissues. In particular this program will fabricate novel anisotropic injectable and printable dense collagen gels with highly defined collagen fibrillar densities and alignments through the use of the newly developed gel aspiration-ejection technique. This technique not only has the ability to control the cellular microenvironment, but can also be scaled-up for the rapid high throughput production of an array of in vitro tissue models for uses in stem cell, toxicology, oncology and neuroscience research and screening. Moreover, when gel aspiration-ejection is applied to cellular scaffolds, cell viability is maintained and the constructs provide a supportive environment for long term cultures to investigate the effects of biochemical and biomechanical cues on tissue maturation. Therefore, the program will continue to generate a number of novel findings on collagenous scaffolds, identifying the roles of various chemical and mechanical cues that impact the functions of both the matrix and three-dimensionally seeded cells aimed at bone tissue engineering, vascularization of tissues, and soft tissue modelling and regeneration. The method is also ideal for generating hybridized scaffolds through the incorporation of other biopolymers or bioinorganics controllably released from so-gel derived soluble and bioactive glasses. The Discovery Grant Program will continue to provide a rich and dynamic environment for the multidisciplinary training of highly qualified personnel. In the previous program six Ph.D. and two M.Eng. students completed their studies. The program will also serve as a platform for innovative technologies that will significantly contribute to tissue engineering, biomaterials development, and to understanding cellular responses to tuneable three dimensional tissue models. Four technologies underwent patent protection applications during the previous program. These have enabled a number of links to be established with glass producing industries, companies targeting medical device and pharma applications as well as research tools and equipment manufacturers, which are expected to grow.

该发现赠款计划的重点是工程学，以及以体外模型开发的密集，组织样胶原式矩阵的结构 - 性能关系的表征，以及用于硬性和软组织再生的支架。特别地，该程序将通过使用新开发的凝胶吸入式技术，制造具有高度定义的胶原蛋白原纤维密度和对齐方式的新型各向异性注射且可打印的致密胶原蛋白凝胶。该技术不仅具有控制细胞微环境的能力，而且还可以缩减用于快速吞吐量的体外组织模型，用于干细胞，毒理学，肿瘤学和神经科学研究和筛查。此外，当将凝胶抽吸量应应用于细胞支架上时，保持细胞活力，构造为长期培养物提供了一个支持环境，以研究生化和生物力学线索对组织成熟的影响。因此，该计划将继续在胶原支架上产生许多新的发现，从而确定影响基质和三维播种细胞的各种化学和机械提示的作用，这些细胞旨在涉及骨组织工程，组织的血管化以及软组织建模和再生。该方法也是通过掺入可从So-Gel衍生的可溶性和生物活性玻璃中释放出来的其他生物聚合物或生物无机物质来产生杂交支架的理想选择。 Discovery Grant计划将继续为高素质人员的多学科培训提供丰富而动态的环境。在上一个课程中，六博士学位和两个M.eng。学生完成了学业。该计划还将作为创新技术的平台，这些平台将极大地有助于组织工程，生物材料的发展以及了解对可调三维组织模型的细胞反应。在上一个计划期间，四种技术接受了专利保护申请。这些已经使许多链接可以通过玻璃生产行业，针对医疗设备和制药应用的公司以及预计将增长的研究工具和设备制造商建立。