Engineered three dimensional tissue-equivalents

工程化三维组织等效物

• 批准号: RGPIN-2016-05363
• 负责人: Nazhat, Showan
• 金额: $ 2.77万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=709667
• 关键词: Engineered; three; dimensional; tissue; equivalents

The focus of this Discovery Grant Program is the engineering, and characterization of the structure-property relationship of dense, tissue-like collagenous matrices developed as in vitro models, and as scaffolds for the regeneration of hard and soft tissues. In particular this program will fabricate novel anisotropic injectable and printable dense collagen gels with highly defined collagen fibrillar densities and alignments through the use of the newly developed gel aspiration-ejection technique. This technique not only has the ability to control the cellular microenvironment, but can also be scaled-up for the rapid high throughput production of an array of in vitro tissue models for uses in stem cell, toxicology, oncology and neuroscience research and screening. Moreover, when gel aspiration-ejection is applied to cellular scaffolds, cell viability is maintained and the constructs provide a supportive environment for long term cultures to investigate the effects of biochemical and biomechanical cues on tissue maturation. Therefore, the program will continue to generate a number of novel findings on collagenous scaffolds, identifying the roles of various chemical and mechanical cues that impact the functions of both the matrix and three-dimensionally seeded cells aimed at bone tissue engineering, vascularization of tissues, and soft tissue modelling and regeneration. The method is also ideal for generating hybridized scaffolds through the incorporation of other biopolymers or bioinorganics controllably released from so-gel derived soluble and bioactive glasses. The Discovery Grant Program will continue to provide a rich and dynamic environment for the multidisciplinary training of highly qualified personnel. In the previous program six Ph.D. and two M.Eng. students completed their studies. The program will also serve as a platform for innovative technologies that will significantly contribute to tissue engineering, biomaterials development, and to understanding cellular responses to tuneable three dimensional tissue models. Four technologies underwent patent protection applications during the previous program. These have enabled a number of links to be established with glass producing industries, companies targeting medical device and pharma applications as well as research tools and equipment manufacturers, which are expected to grow.

这一发现资助计划的重点是对致密的组织样胶原基质的结构-性能关系进行工程设计和表征，这些基质被开发为体外模型，并作为硬组织和软组织再生的支架。特别是，该计划将通过使用最新开发的凝胶吸入-喷射技术来制造具有高度定义的胶原纤维密度和排列的新型各向异性、可注射和可打印的致密胶原蛋白凝胶。这项技术不仅具有控制细胞微环境的能力，而且还可以扩大规模，快速高通量地生产一系列用于干细胞、毒理学、肿瘤学和神经科学研究和筛选的体外组织模型。此外，当凝胶吸入-喷射应用于细胞支架时，细胞活力保持不变，构建物为长期培养提供了支持环境，以研究生化和生物力学信号对组织成熟的影响。因此，该计划将继续产生许多关于胶原支架的新发现，确定各种化学和机械线索的作用，这些线索影响旨在骨组织工程、组织血管化以及软组织建模和再生的基质和三维种子细胞的功能。该方法也是通过加入其他生物聚合物或生物无机物来生成杂化支架的理想方法，这些生物聚合物或生物无机物可控制地从凝胶衍生的可溶性和生物活性玻璃中释放出来。 探索助学金计划将继续为高素质人才的多学科培训提供丰富和充满活力的环境。在上一个项目中，六名博士和两名工程硕士。学生们完成了学业。该计划还将作为创新技术的平台，这些技术将对组织工程、生物材料开发以及了解细胞对可调三维组织模型的反应做出重大贡献。在之前的计划中，有四项技术接受了专利保护申请。这使得与玻璃生产行业、瞄准医疗设备和制药应用的公司以及研究工具和设备制造商建立了许多联系，这些公司预计将会增长。