"Multimodal Optical Imaging Endoscopy for Noninvasive ""Optical Biopsy"" in Tissues"

“用于组织中无创“光学活检”的多模态光学成像内窥镜”

• 批准号: 341640-2012
• 负责人: Tang, Shuo
• 金额: $ 1.53万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=572329
• 关键词: Multimodal; Optical; Imaging; Endoscopy; Noninvasive

Lung cancer is the foremost cause of cancer death in Canada. In 2011, lung cancer is estimated to account for 11,300 of cancer deaths in men and 9,300 in women. Early detection and treatment has the potential to greatly improve these statistics. The current gold standard of cancer diagnosis is tissue biopsy followed by histopathology. In this procedure, clinicians take multiple tissue biopsies from suspicious areas and perform histopathology examination. This procedure is invasive and time consuming. "Optical biopsy" is a new type of tool which uses optical imaging to examine subcellular tissue morphology in vivo. It can provide the same imaging resolution as histopathology but the procedure is noninvasive and in real-time. Therefore, "optical biopsy" can potentially have a huge impact on the diagnosis and treatment of cancer and other diseases. We propose to develop a multimodal optical imaging endoscopy for noninvasive "optical biopsy" of the lung. The endoscope will combined optical coherence tomography (OCT) and multiphoton microscopy (MPM). OCT provides cross-sectional anatomical imaging of layered tissue structures over a few millimeters deep and wide. It detects backscattered light from refractive index discontinuities. MPM images cells and extracellular matrix in turbid tissues with subcellular resolution. MPM provides functional information about biochemical specificity of collagen, elastin, NADH, and flavins. When developed into a single endoscope, MPM/OCT can provide multiple contrasts and multiscale field-of-views. The proposal will develop endoscopic MPM/OCT using optical fibers, miniature scanners, and micro-optics. The design will be optimized for simultaneous large field-of-view OCT and high resolution MPM imaging. The endoscope will be capable to quickly scan over a large tissue area to find abnormal regions with OCT, and perform a high-resolution zoom-in imaging on the regions of interest to identify the abnormalities with MPM. The development from this project will serve as an important tool for diagnosing and evaluating the effect of treatment of major respiratory diseases.

肺癌是加拿大癌症死亡的首要原因。 2011 年，据估计，肺癌导致男性癌症死亡人数为 11,300 人，女性癌症死亡人数为 9,300 人。早期发现和治疗有可能大大改善这些统计数据。目前癌症诊断的金标准是组织活检，然后进行组织病理学检查。在此过程中，临床医生从可疑区域采集多个组织活检并进行组织病理学检查。这个过程是侵入性的并且耗时。 “光学活检”是一种利用光学成像检查体内亚细胞组织形态的新型工具。它可以提供与组织病理学相同的成像分辨率，但该过程是无创且实时的。因此，“光学活检”可能对癌症和其他疾病的诊断和治疗产生巨大影响。 我们建议开发一种多模态光学成像内窥镜，用于肺部无创“光学活检”。该内窥镜将结合光学相干断层扫描（OCT）和多光子显微镜（MPM）。 OCT 提供深度和宽度超过几毫米的分层组织结构的横截面解剖成像。它检测来自折射率不连续性的反向散射光。 MPM 以亚细胞分辨率对混浊组织中的细胞和细胞外基质进行成像。 MPM 提供有关胶原蛋白、弹性蛋白、NADH 和黄素的生化特异性的功能信息。当开发成单一内窥镜时，MPM/OCT 可以提供多种对比度和多尺度视场。该提案将使用光纤、微型扫描仪和微光学器件开发内窥镜 MPM/OCT。该设计将针对同步大视场 OCT 和高分辨率 MPM 成像进行优化。内窥镜将能够快速扫描较大的组织区域，以通过 OCT 发现异常区域，并通过 MPM 对感兴趣区域进行高分辨率放大成像以识别异常区域。该项目的开发将成为诊断和评估重大呼吸系统疾病治疗效果的重要工具。