Handheld Multiphoton Imaging Device for Detecting Oral Cancer

根据加拿大癌症协会的数据，口腔癌的五年存活率只有**63%。加拿大每年新增病例4600例，全球新增病例30万例，**平均有一半患者在确诊后5年内死亡。口腔癌术后复发率高达20%。因此，迫切需要能够改进口腔癌早期发现和治疗的新技术。在这个项目中，将开发一种新的基于多光子成像的癌症成像设备，以提供活体和实时的高分辨率口腔粘膜病变成像。多光子成像可以为癌症检测提供重要的生物标志物的可视化：肿瘤细胞和细胞核的形态变化**，以及促进肿瘤细胞进展和侵袭的潜在胶原纤维的密度和有序。在动物模型的临床前癌症研究中，它被证明是非常**成功的。将多光子成像**转化为临床应用才刚刚开始。挑战包括处理飞秒脉冲**和有限的信噪比。我们将解决这些挑战，并设计一种便携式**多光子系统**手持探头，用于对口腔粘膜进行活体成像。**将在患者身上进行体内成像，以评估该设备用于引导活检和**肿瘤边缘检测。这项新技术可以通过对肿瘤进行实时非侵入性评估，并指导决定在何时何地进行活检以早期发现癌症，从而改善癌症管理。

According to the Canadian Cancer Society, the 5-year survival rate for oral cancer is only**63%. Each year 4,600 new cases in Canada and 300,000 cases worldwide are identified and**on average, half of the patients die within 5 years of diagnosis. The recurrence rate of oral**cancer after surgery is high at 20%. Therefore, there is a critical need for new technology that**can improve the early detection and treatment of oral cancer. In this project, a new point-ofcare**cancer imaging device based on multiphoton imaging will be developed to provide highresolution**imaging of oral mucosa lesions in vivo and in real-time. Multiphoton imaging can**provide visualization of important biomarkers for cancer detection: the morphological changes**of tumor cells and nuclei, and the density and ordering of the underlying collagen fibers which**facilitates the progression and invasion of tumor cells. It has been demonstrated to be very**successful in pre-clinical cancer studies on animal models. Translating multiphoton imaging**into clinical applications is just beginning. Challenges include handling femtosecond pluses**and limited signal to noise ratio. We will address those challenges and design a portable**multiphoton system with a handheld probe for performing in vivo imaging of the oral mucosa.**In vivo imaging on patients will be carried out to evaluate the device for guided biopsy and**tumor margin detection. The new technology can improve cancer management by real-time**assessment of tumor noninvasively and guiding decisions on when and where to take**biopsies for early cancer detection.