Microplate based method for the detection of biocides in wastewater

基于微孔板的废水中杀菌剂检测方法

• 批准号: 484193-2015
• 负责人: Filipe, Carlos
• 金额: $ 1.82万
• 依托单位: McMaster University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=584170
• 关键词: Microplate; based; method; detection; biocides

Currently, Aevitas Inc does not have rapid screening test for the wastewater that is brought in for treatment at its facilities. This means that under current conditions, the testing process takes 6 hours, and the test results are often unreliable. In order to be competitive and keep up with the regulations of the City of Brantford, Aevitas requires the ability to quickly and accurately screen wastewater coming into their treatment facilities for the presence of biocides, since this will determine the best strategy for treating that waste and the associated costs to their customers. The McMaster research team will develop screening method that will determine the presence of biocides in samples provided by Aevitas by having a pre-measured amount of bacteria, exposing them to the wastewater and determining the viability of the bacteria by measuring the amount of ATP (adenosine triphosphate) in these bacteria as a function of the waste concentration. This will enable Aevitas to determine the appropriate treatment.

目前，Aevitas Inc.没有对被带到其设施进行处理的废水进行快速筛选测试。这意味着，在目前条件下，检测过程需要6个小时，检测结果往往不可靠。为了具有竞争力并跟上布兰特福德市的法规，Aevitas需要能够快速准确地筛选进入其处理设施的废水中是否存在杀菌剂，因为这将决定处理该废物的最佳策略及其客户的相关成本。麦克马斯特研究小组将开发一种筛选方法，通过预先测量细菌数量，将它们暴露在废水中，并通过测量三磷酸腺苷的量来确定细菌的活性，从而确定Aevitas提供的样本中是否存在杀生剂 (三磷酸腺苷)在这些细菌中作为废物浓度的函数。这将使Aevitas能够确定适当的治疗方法。