Impact of TRPV6 ligation on colonic epithelial cell-dendritic cell crosstalk during induction of an innate immune response

TRPV6 连接对诱导先天免疫反应期间结肠上皮细胞-树突状细胞串扰的影响

• 批准号: 488435-2015
• 负责人: Hoskin, David
• 金额: $ 1.82万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=586247
• 关键词: Impact; TRPV6; ligation; colonic; epithelial

Recent findings have established the importance of communication between the epithelial cells that line the gut and dendritic cells, which are a type of white blood cell, for initiating and regulating host immune responses. One of the molecules that might be involved in these important interactions is a calcium channel, termed TRPV6, that has signaling properties and is expressed by epithelial cells in the colon. Soricimed Biopharma Incorporated (SBI) has developed a family of small proteins that bind to and alter the activity of TRPV6, and is interested to determine whether binding of these proteins to colonic epithelial cells affects the ability of nearby dendritic cells to response to a bacterial "danger signal". In this collaborative research project, we will determine the effect of TRPV6-selective peptide interactions on the ability of dendritic cells to produce various chemical messengers response to CpG oligodeoxynucleotide present in the DNA of bacteria. This collaboration represents a unique opportunity to explore the response of dendritic cells to "danger signals" in the context of altered TRPV6 calcium channel function of gut epithelial cells.

最近的研究结果证实了肠道上皮细胞之间通讯的重要性 树突状细胞是白细胞的一种，用于启动和调节宿主免疫反应。 可能参与这些重要相互作用的分子之一是钙通道，称为 TRPV6 具有信号传导特性，由结肠上皮细胞表达。索瑞美德生物制药 Incorporated (SBI) 开发了一系列小蛋白，可结合并改变 TRPV6 的活性，并且 有兴趣确定这些蛋白质与结肠上皮细胞的结合是否会影响附近的能力 树突状细胞对细菌的“危险信号”做出反应。在这个合作研究项目中，我们将 确定 TRPV6-选择性肽相互作用对树突状细胞产生各种物质的能力的影响 化学信使对细菌 DNA 中存在的 CpG 寡脱氧核苷酸做出反应。此次合作 代表了探索树突状细胞对“危险信号”的反应的独特机会 改变肠上皮细胞的 TRPV6 钙通道功能。