Development of microbial inoculant formulations for controlling wireworms and increasing potato productivity

许多生活在植物根际（即靠近根部的土壤）的有益细菌可以 促进植物生长和/或表现出抗微生物和杀虫活性。这些生物被称为 植物生长促进根际细菌（PGPR），可能属于不同的分类群，包括 假单胞菌属和芽孢杆菌属。假单胞菌属和芽孢杆菌属的一些菌株能刺激植物生长 直接和/或间接地通过各种机制。其中一些菌株还产生广谱 抗微生物和杀虫化合物。假单胞菌属和芽孢杆菌属是理想的候选者， 和/或减少化肥和杀虫剂的使用，因为它们已经参与了自然平衡 植物和环境之间的关系，它们可以很容易地培养和接种， 是化肥和农药应用的合理替代品。在PGPR中， 衍生品生产商的利益很高，因为他们广泛的对抗行动，对广泛的 病原体和害虫，以及它们的植物生长促进能力。费博士的研究小组在 蒙克顿大学已经分离出大量生产吩嗪-1-羧酸的假单胞菌菌株 (PCA)并且已经广泛地表征了这些细菌的植物生长促进和生物防治 能力的在这个项目中，Festival博士的研究小组分离出了两种有希望的PCA生产菌株， 荧光假单胞菌LBUM 223和荧光假单胞菌LBUM 636，它们是马铃薯植物生长促进剂， 将测试它们在单独接种或与以下组合接种时控制马铃薯线虫的能力： 芽孢杆菌我们的商业合作伙伴Nuvac Ecoscience已经将菌株商业化。线虫是 昆虫的幼虫，在进食时钻入块茎时会造成很深的洞，从而损坏马铃薯。 利用新型微生物菌剂开发防治线虫的环保产品 新的配方将对马铃薯生产力和环境产生积极影响。

Many beneficial bacteria living in the rhizosphere of plants (i.e. the soil in close proximity to roots) can promote plant growth and/or exhibit antimicrobial and insecticidal activity. These organisms are known as plant growth promoting rhizobacteria (PGPR) and may belong to different taxonomical groups, including Pseudomonas sp. and Bacillus sp. Some strains of Pseudomonas sp. and Bacillus sp. can stimulate plant growth directly and/or indirectly by various mechanisms. Some of these strains also produce broad spectrum antimicrobial and insecticidal compounds. Pseudomonas sp. and Bacillus sp. are ideal candidates for replacing and/or reducing the use of chemical fertilizers and pesticides as they already take part in the natural equilibrium between plants and the environment, they can be easily cultured and inoculated, and their production cost represents a reasonable alternative to chemical fertilizer and pesticide applications. Among PGPR, phenazine derivative-producers are of high interest due to their broad antagonistic actions against a wide range of pathogens and pests, as well as their plant growth promoting capabilities. Dr. Filion's research team at the Université de Moncton has isolated numerous strains of Pseudomonas sp. producing phenazine-1-carboxylic (PCA) and has extensively characterized these bacteria for their plant growth promoting and biocontrol capabilities. In this project, two promising PCA-producing strains isolated by Dr. Filion's research team, Pseudomonas fluorescens LBUM223 and P. fluorescens LBUM636, which are potato plant growth promoters, will be tested for their capacity at controlling potato wireworms when inoculated alone or in combination with Bacillus spp. strains already commercialized by our commercial partner, Nuvac Eco-science. Wireworms are insects larvae that damage potatoes by creating deep holes when burrowing into tubers while feeding. Developing an environmentally-friendly product to control wireworms, using novel microbial inoculant formulations, will have a positive impact on potato productivity as well as the environment.