Characterization of the microbial diversity found under cannabis production conditions

LuminUltra是一家私营公司，成立于新泽西州弗雷德里克顿，开发并推向市场**微生物检测和表征技术。为了进一步使其产品线多样化，LuminUltra要求我帮助开发新的基于dna的试剂盒和技术，旨在检测和量化大麻行业感兴趣的微生物。**加拿大的大麻产业目前正在蓬勃发展，持牌生产商正在寻求外部专业知识，以检测和量化**商业大麻生产条件下存在的感兴趣的微生物**（微生物植物病原体、人类病原体、有益植物有益微生物等）。因此，在这个项目中，我提议与我的合作伙伴LuminUltra合作：1)表征大麻根际和层际微生物组；2)根据目标1获得的结果，鉴定目标微生物，用于开发用于商业大麻生产条件下的特定**监测定量生物测定。该项目将有助于更好地了解大麻植物的微生物组，并为开发新的定量监测技术确定微生物靶点。在商业大麻生产条件下跟踪其中一些关键微生物，将对提高植物生产力、**确保采取适当的植物疾病控制措施、防止人类病原体疾病爆发**和确保对大麻产品进行最佳质量控制**产生积极影响。

LuminUltra, a privately owned company established in Fredericton, NB, develops and brings to market**microbial detection and characterization technologies. In an effort to further diversify their line of products,**LuminUltra has requested my help to develp novel DNA-based kits and technologies aiming at detecting and**quantifying microorganisms of interest to the Cannabis industry. The cannabis industry is presently booming in**Canada and licensed producers are seeking external expertise to detect and quantify microorganisms of interest**(microbial plant pathogens, human pathogens, beneficial plant-beneficial microorganisms, etc.) present under**commercial cannabis production conditions. In this project, I therefore propose, in collaboration with my**partner LuminUltra, to: 1) characterize the cannabis rhizosphere and phyllosphere microbiomes; and 2)**identify, based on the results obtained in objective 1, target microorganisms for the development of specific**monitoring quantitative bioassays for use under commercial cannabis production conditions. This project will**help better understand the microbiome of the cannabis plant, as well as identify microbial targets for the**development of novel quantitative monitoring technologies. Tracking some of these key microorganisms under**commercial cannabis production conditions will have a positive impact on improving plant productivity,**ensuring appropriate plant disease control measures, as well as preventing human pathogen disease outbreaks**and ensuring optimal quality control of cannabis products.