Cannabis plant growth promotion using PGPR

使用 PGPR 促进大麻植物生长

• 批准号: 513714-2017
• 负责人: Filion, Martin
• 金额: $ 1.82万
• 依托单位: Université de Moncton
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=639321
• 关键词: Cannabis; plant; growth; promotion; using

Many beneficial bacteria living in the rhizosphere of plants (i.e. the soil in close proximity to roots) canpromote plant growth. These organisms are known as plant growth promoting rhizobacteria (PGPR) and maybelong to different taxonomical groups, including Pseudomonas sp. and Bacillus sp. Some strains ofPseudomonas sp. and Bacillus sp. can stimulate plant growth directly and/or indirectly by various mechanisms.Pseudomonas sp. and Bacillus sp. are ideal candidates for replacing and/or reducing the use of chemicalfertilizers as they can be easily cultured and inoculated, and their production cost represents a reasonablealternative to chemical fertilizer applications. Although numerous strains of Pseudomonas sp. and Bacillus sp.have been identified as potential PGPR in different systems, combinations of Pseudomonas sp./Bacillus sp.strains that can synergistically impact on plant growth have not been widely studied. Over the years, myresearch team has isolated and characterized numerous strains of Pseudomonas sp. for their plant growthpromoting capacities. In parallel, Biotechnology Ulysse has developed a large expertise in isolating andcharacterizing Bacillus sp. strains for plant growth promotion. Therefore, our expertise is complimentary andcan lead to the development of microbial consortia that display superior synergistic plant growth promotingactivity. Biotechnologies Ulysse is seeking help to be among the first Canadian companies able tocommercialize environmentally-friendly microbial-based fertilizers specifically tailored to the Cannabisindustry. Therefore, in this project, a collection of 60 different Pseudomonas sp strains isolated in ourlaboratory will be tested in combination with two Bacillus sp. strains (owned by Biotechnologies Ulysse) fortheir capacity at promoting Cannabis growth and productivity. More specifically, the aims are to: 1) Identify themost optimal Pseudomonas sp./Bacillus sp. combination capable of promoting Cannabis plant growth; and 2)Develop specific DNA molecular markers for these organisms allowing to detect and quantify their populationdynamics under soil and plant conditions using qPCR assays.

许多生活在植物根际（即靠近根部的土壤）的有益细菌能促进植物生长.这些微生物被称为植物根际促生菌（PGPR），可能属于不同的分类群，包括假单胞菌属（Pseudomonas sp.）和芽孢杆菌属（Bacillus sp.）。假单胞菌属（Pseudomonas sp.）和芽孢杆菌属（Bacillus sp.）的一些菌株可以通过各种机制直接和/或间接刺激植物生长。假单胞菌属（Pseudomonas sp.）和芽孢杆菌属（Bacillus sp.）是替代和/或减少化学肥料使用的理想候选者，因为它们易于培养和接种，并且它们的生产成本代表了化肥应用的合理替代。虽然假单胞菌属和芽孢杆菌属的许多菌株已被鉴定为在不同系统中的潜在PGPR，但假单胞菌属/芽孢杆菌属的组合在不同的系统中是潜在的PGPR。能够协同影响植物生长的芽孢杆菌菌株尚未被广泛研究。多年来，我的研究小组已经分离和鉴定了许多假单胞菌菌株，以了解它们促进植物生长的能力。与此同时，Biotechnology Ulysse在分离和鉴定促进植物生长的芽孢杆菌菌株方面积累了丰富的专业知识。因此，我们的专业知识是互补的，可以导致微生物财团的发展，显示上级协同植物生长促进活动。生物技术Ulysse正在寻求帮助，成为首批能够将专为大麻行业量身定制的环保微生物肥料商业化的加拿大公司之一。因此，在本项目中，我们实验室分离的60种不同的假单胞菌菌株将与两种芽孢杆菌菌株（由Biotechnologies Ulysse拥有）结合测试其促进大麻生长和生产力的能力。更具体地说，目的是：1）鉴定最佳假单胞菌属。芽孢杆菌组合能够促进大麻植物生长;和2）开发这些生物体的特异性DNA分子标记，允许使用qPCR测定来检测和量化它们在土壤和植物条件下的种群动态。