Molecular characterization of bacterial antibiosis in the rhizosphere and its impact on Solanaceous plant diseases

根际细菌抗菌的分子特征及其对茄科植物病害的影响

• 批准号: 283254-2011
• 负责人: Filion, Martin
• 金额: $ 1.97万
• 依托单位: Université de Moncton
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=594799
• 关键词: Molecular; characterization; bacterial; antibiosis; rhizosphere

An increased interest in biocontrol of plant pathogens has been fuelled by public concern over the use of chemical pesticides in the environment and ineffective chemical control measures against certain diseases. It has been demonstrated that several antagonistic Pseudomonas spp. can control plant pathogen populations under in vitro or controlled conditions using antibiosis. However, the efficiency of antibiosis in soil is still variable, explaining why only few antimicrobial metabolite-producing Pseudomonas spp. strains have been commercialized as biopesticides. Here, it is proposed that two Solanaceous plant species, potato and tomato, be used to study the effect of tailored antimicrobial metabolite-producing Pseudomonas spp. inoculations against two important bacterial plant pathogens: Streptomyces scabiei (Ss) causing common scab of potato and Clavibacter michiganensis subsp. michiganensis (Cmm) causing bacterial wilt and canker of tomato. The general goal of this research is to understand, at the molecular level, how antibiosis operates in the rhizosphere, how it affects the transcriptomics of Ss and Cmm, its effect on colonization and infection of plants by pathogens, and how it can protect against diseases. A collection of Pseudomonas spp. producing antimicrobial metabolites such as phenazine-1-carboxylic acid (PCA), 2,4-diacetylphloroglucinol (DAPG) and hydrogen cyanide (HCN) has been isolated in our laboratory. Knockouts of these strains, incapable of producing the antimicrobial metabolites, have also been developed. These organisms will be used to address the following specific goals: 1) Characterize the transcriptional activity associated with Pseudomonas spp. antimicrobial metabolite production in the rhizosphere of potato and tomato and its impact on controlling plant diseases. 2) Study the impact of antimicrobial metabolite production on Ss and Cmm transcriptomics. 3) Determine if antimicrobial metabolite production can, in addition to antibiosis, trigger induced systemic resistance (ISR) in Solanaceae. This innovative work should lead to a better understanding of how Pseudomonas spp.-mediated antibiosis operates under soil conditions and how it can contribute to disease protection in Solanaceae.

公众对在环境中使用化学农药的使用以及针对某些疾病的无效化学控制措施的关注激发了人们对植物病原体生物防治的兴趣的增加。已经证明了几个拮抗假单胞菌属。可以在体外或受控条件下使用抗生素控制植物病原体种群。但是，土壤中抗体症的效率仍然可变，这解释了为什么只有少数抗菌代谢物生成假单胞菌属的效率。菌株已被商业化为生物农药。在这里，建议使用两种静电植物物种，马铃薯和番茄来研究量身定制的抗菌代谢物生产假单胞菌的作用。对两种重要的细菌植物病原体的接种：链霉菌（SS），导致马铃薯和clavibacter michiganensis subsp的常见scab。密歇根州（CMM）引起番茄的细菌枯萎病和溃疡。这项研究的一般目标是在分子水平上了解抗体中毒在根际中的作用，如何影响SS和CMM的转录组学，其对病原体对植物的定殖和感染的影响以及如何保护疾病。假单胞菌属的集合。在我们的实验室中分离了产生抗菌代谢物，例如苯并氨酸-1-羧酸（PCA），2,4-二乙基氯糖醇（DAPG）和氰化氢（HCN）。这些菌株的敲除也无法产生抗菌代谢物。这些生物将用于解决以下特定目标：1）表征与假单胞菌属相关的转录活动。马铃薯和番茄根际中的抗菌代谢产物及其对控制植物疾病的影响。 2）研究抗菌代谢物的产生对SS和CMM转录组学的影响。 3）确定除抗抗病性外，抗菌代谢物的产生是否还可以触发溶剂科诱导的全身耐药性（ISR）。这项创新的工作应该可以更好地理解假单胞菌如何在土壤条件下进行抗生素作用，以及它如何为茄科中的疾病保护做出贡献。