Phenazine-producing Pseudomonas spp. in the rhizosphere

产吩嗪假单胞菌属。

基本信息

批准号：
RGPIN-2018-05217
负责人：
Filion, Martin
金额：
$ 3.42万
依托单位：
Université de Moncton
依托单位国家：
加拿大
项目类别：
Discovery Grants Program - Individual
财政年份：
2019
资助国家：
加拿大
起止时间：
2019-01-01 至 2020-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=689547
关键词：
Phenazine producing Pseudomonas spp.rhizosphere

项目摘要

Pseudomonas spp. are aerobic, Gram-negative bacteria ubiquitously found in soils. They are particularly well suited for plant root colonization and many plant-beneficial strains display biocontrol activity against plant pathogens through various mechanisms, including antibiosis. Among the wide array of antibiotics produced by plant-beneficial Pseudomonas spp., phenazines display broad-spectrum antimicrobial activity. Different phenazine derivatives have been shown to play a crucial role in the biocontrol of several pathogens in various plant systems as well as being involved in rhizocompetence (i.e. the capacity to colonize the rhizosphere) and biofilm formation in the rhizosphere. ******Plant-beneficial Pseudomonas spp. possess a large genome which displays a mosaic structure with genes segregating between the core genome, responsible for essential cellular processes, and the accessory genome, often unique to one or few strains, and responsible for the variability observed among strains. The genomic diversity and plasticity of plant-beneficial Pseudomonas spp. come from their accessory genomes. Recently, our laboratory assembled the largest worldwide collection of plant-beneficial phenazine-producing Pseudomonas spp. A collection of 63 representative organisms isolated from 18 countries were chosen to account for the large diversity of host plants and environments from where they were isolated, their worldwide distribution, their taxonomical diversity, and the array of phenazine derivatives produced. The sequencing and annotation of their genomes has recently been completed by our laboratory. This unique large data set will be used as a starting point for this research project. The primary goal of this research is to better characterize and understand how plant-beneficial phenazine-producing Pseudomonas spp. influence the ecology of the rhizosphere, leading to improved plant health, and protection against various plant diseases. The specific goals are to: ******1) Characterize and compare the worldwide diversity of plant-beneficial phenazine-producing Pseudomonas spp. at the genomics and functional levels. ***2) Characterize and contrast the mechanisms employed by plant-beneficial phenazine-producing Pseudomonas spp. against bacterial, oomycete and fungal plant pathogens. ***3) Identify key genetic determinants in plant-beneficial phenazine-producing Pseudomonas spp. involved in rhizocompetence.******A better understanding of the diversity, ecology and genomics of plant-beneficial phenazine-producing Pseudomonas spp., as well as the mechanisms involved in their rhizocompetence and biocontrol of plant pathogens is required to ensure the successful development, formulation and commercialisation of some of these organisms as biopesticides. This research program will address this gap in knowledge.**

假单胞菌属。是有氧，革兰氏阴性细菌普遍存在的土壤中。它们特别适合植物根定植，许多植物菌菌株通过包括抗生素（包括抗生素）的各种机制对植物病原体表现出生物防治活性。在由植物 - 苯酚假单胞菌属产生的广泛的抗生素中，促苯胺表现出广泛的抗菌活性。已经显示，不同的苯嗪衍生物在各种植物系统中的多种病原体的生物防治中起着至关重要的作用，并且参与根茎中的根茎（即实现根际）和生物膜形成的能力。 ******植物 - 苯基假单胞菌属。拥有一个大基因组，该基因组显示出镶嵌结构，其基因在核心基因组之间隔离，负责基本细胞过程和辅助基因组，而辅助基因组通常是一个或几种菌株独有的，并且负责菌株之间观察到的可变性。植物伴有假单胞菌属的基因组多样性和可塑性。来自他们的附属基因组。最近，我们的实验室组装了全球最大的植物苯并苯嗪产生假单胞菌属的集合。选择了从18个国家隔离的63种代表性生物的集合来解释寄主植物和环境的众多多样性，其全球分布，其分类学多样性以及生产的苯嗪衍生物阵列。我们的实验室最近完成了其基因组的测序和注释。这个独特的大数据集将用作该研究项目的起点。这项研究的主要目的是更好地表征和了解植物性苯嗪产生假单胞菌属的方式。影响根际的生态学，从而改善植物健康，并保护各种植物疾病。具体目标是：****** 1）表征和比较了植物呈苯嗪产生假单胞菌属的全球多样性。在基因组学和功能水平上。 *** 2）表征和对比由产生苯嗪的假单胞菌spp所采用的机制。针对细菌，卵巢和真菌植物病原体。 *** 3）确定产生苯嗪苯嗪假单胞菌属的关键遗传决定因素。 ******对产生苯丙胺的多样性，生态和基因组学的多样性，生态学和基因组学的理解以及植物病原体的根茎和生物控制所涉及的机制需要确保某些有机化的成功开发，形成和商业化。该研究计划将解决知识的差距。**