Upgrade of Spinning Disk Confocal Microscope

转盘共焦显微镜的升级

• 批准号: RTI-2016-00099
• 负责人: Samuels, AnneLacey
• 金额: $ 10.78万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591343
• 关键词: Upgrade; Spinning; Disk; Confocal; Microscope

The Perkin-Elmer (P-E) Ultraview VoX spinning disk confocal microscope is a workhorse instrument for detecting fluorescent proteins, such as green fluorescent protein (GFP), in living cells. We use the P-E Spinning Disk confocal microscope in studies of how cellulose is made in plant cells, for examining plant cells during growth and development, and for investigating plants' and animals' responses to stress. The current microscope is seven years old, and it is beginning to fall behind international standards due to its outdated camera, broken stage (sample holder), and aging lasers. Because the current microscope is outdated, it cannot be used for live cell imaging for very rapid events. As this workhorse microscope ages, we are increasingly facing problems. In order to collect the data for his Ph.D., and his subsequent Science paper, UBC Botany student Yoshi Watanabe was forced to go to Stanford University to use their spinning disk confocal. Our spinning disk requires upgrades to the laser, the stage, the optics, and the camera. The microscope is housed in, and managed by, the UBC Bioimaging Facility, a campus-wide biological imaging facility. UBC Bioimaging Facility provides top-end light and electron microscopy infrastructure to more than 100 research groups from 25 UBC departments. Upgrades to the spinning disk confocal will benefit many HQP from across campus. There were 30 HQP using the microscope in 2014-2015, from Botany (Samuels, Haughn, Douglas, Kunst, Wasteneys labs), Forestry (Mansfield, Bohlmann labs), Pediatrics (Maxwell lab), Microbiology and Immunology (Hancock lab), Chemistry (Perrin lab), and Zoology (Brauner, Milsom, Auld labs). The many on-going projects that rely on this equipment generate the urgency of this request.

Perkin-Elmer（P-E）Ultraview Vox旋转磁盘共聚焦显微镜是一种用于检测活细胞中荧光蛋白（例如绿色荧光蛋白（GFP））的主力仪器。我们在研究植物细胞中如何制作纤维素，在生长和发育过程中检查植物细胞的研究以及研究植物和动物对压力的反应。当前的显微镜已经使用了七年，由于其过时的相机，破碎的舞台（样品持有人）和衰老激光器，它开始落后国际标准。由于当前的显微镜已过时，因此不能用于实时细胞成像进行非常快速的事件。随着这种主力显微镜的年龄，我们越来越面临问题。为了收集其博士学位的数据以及他随后的科学论文，UBC植物学学生Yoshi Watanabe被迫去斯坦福大学使用旋转磁盘共享。我们的旋转磁盘需要升级到激光器，舞台，光学器件和相机。显微镜被安置在校园范围内的生物成像设施中，并由UBC生物成像设施进行管理。 UBC生物成像设施为来自25个UBC部门的100多个研究组提供了高端光和电子显微镜基础设施。升级到旋转磁盘共焦的升级将使许多来自校园的HQP受益。 There were 30 HQP using the microscope in 2014-2015, from Botany (Samuels, Haughn, Douglas, Kunst, Wasteneys labs), Forestry (Mansfield, Bohlmann labs), Pediatrics (Maxwell lab), Microbiology and Immunology (Hancock lab), Chemistry (Perrin lab), and Zoology (Brauner, Milsom, Auld实验室）。 许多依赖此设备的正在进行的项目都会产生此请求的紧迫性。