Upgrade of Spinning Disk Confocal Microscope

转盘共焦显微镜的升级

• 批准号: RTI-2016-00099
• 负责人: Samuels, AnneLacey
• 金额: $ 10.78万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591343
• 关键词: Upgrade; Spinning; Disk; Confocal; Microscope

The Perkin-Elmer (P-E) Ultraview VoX spinning disk confocal microscope is a workhorse instrument for detecting fluorescent proteins, such as green fluorescent protein (GFP), in living cells. We use the P-E Spinning Disk confocal microscope in studies of how cellulose is made in plant cells, for examining plant cells during growth and development, and for investigating plants' and animals' responses to stress. The current microscope is seven years old, and it is beginning to fall behind international standards due to its outdated camera, broken stage (sample holder), and aging lasers. Because the current microscope is outdated, it cannot be used for live cell imaging for very rapid events. As this workhorse microscope ages, we are increasingly facing problems. In order to collect the data for his Ph.D., and his subsequent Science paper, UBC Botany student Yoshi Watanabe was forced to go to Stanford University to use their spinning disk confocal. Our spinning disk requires upgrades to the laser, the stage, the optics, and the camera. The microscope is housed in, and managed by, the UBC Bioimaging Facility, a campus-wide biological imaging facility. UBC Bioimaging Facility provides top-end light and electron microscopy infrastructure to more than 100 research groups from 25 UBC departments. Upgrades to the spinning disk confocal will benefit many HQP from across campus. There were 30 HQP using the microscope in 2014-2015, from Botany (Samuels, Haughn, Douglas, Kunst, Wasteneys labs), Forestry (Mansfield, Bohlmann labs), Pediatrics (Maxwell lab), Microbiology and Immunology (Hancock lab), Chemistry (Perrin lab), and Zoology (Brauner, Milsom, Auld labs). The many on-going projects that rely on this equipment generate the urgency of this request.

Perkin-Elmer(P-E)Ultraview Vox旋转圆盘共聚焦显微镜是检测活细胞中绿色荧光蛋白(GFP)等荧光蛋白的主要仪器。我们使用P-E旋转盘共聚焦显微镜研究植物细胞中的纤维素是如何产生的，用于检查植物细胞在生长和发育过程中的情况，以及研究植物和动物对胁迫的反应。目前的显微镜已经有七年的历史了，由于相机过时、工作台(样品架)损坏和激光老化，它开始落后于国际标准。由于目前的显微镜已经过时，它不能用于非常快速的事件的活细胞成像。随着这台主力显微镜的老化，我们面临的问题也越来越多。为了收集博士论文和随后发表的《科学》论文的数据，UBC植物学专业的学生渡边义士被迫去斯坦福大学使用他们的旋转磁盘共焦。我们的旋转磁盘需要升级激光、舞台、光学设备和相机。显微镜被安置在UBC生物成像设施中，并由该设施管理，UBC生物成像设施是一家校园范围的生物成像设施。UBC生物成像设备为来自25个UBC部门的100多个研究小组提供高端光学和电子显微镜基础设施。对旋转磁盘共焦的升级将使园区内的许多HQP受益。在2014-2015年间，使用显微镜的HQP有30个，分别来自植物学(Samuels，Haughn，Douglas，Kost，Wasteneys Lab)，林业(Mansfield，Bohlmann Lab)，儿科(Maxwell Lab)，微生物学和免疫学(Hancock Lab)，化学(Perrin Lab)和动物学(Brauner，Milsom，Auld Lab)。依赖这种设备的许多正在进行的项目产生了这一要求的紧迫性。