Live Cell Imaging Platform for Dynamic Studies of Cellular Interactions

用于细胞相互作用动态研究的活细胞成像平台

基本信息

批准号：
RTI-2016-00466
负责人：
Zhang, Shetuan
金额：
$ 10.34万
依托单位：
Queen's University
依托单位国家：
加拿大
项目类别：
Research Tools and Instruments
财政年份：
2015
资助国家：
加拿大
起止时间：
2015-01-01 至 2016-12-31
项目状态：
已结题

来源：
https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591449
关键词：
Live Cell Imaging Platform Dynamic

项目摘要

The current proposal supports the growing need of an emerging group of cell biology researchers at Queen’s University to access imaging platforms designed to actively track cellular phenotypes in live cells. The IncuCyte ZOOM imaging system will provide these research groups with tools to track cells as they divide, move, interact with other cells, and undergo programmed cell death. This imaging platform will facilitate new discoveries in studies of cells isolated from a variety of tissues (heart, brain, breast, gastrointestinal tract, peripheral blood). The parallel study of hundreds of cell samples in this instrument is a significant advance over current imaging systems. The system has been developed for imaging of both unlabeled cells, as well as with fluorescent signals that provide information on the cell phenotypes within live cells. It will also allow Queen's investigators to perform high throughput screening and dose response studies that are needed to develop new tools to uncover the molecular basis of cell movement, cell-cell interactions, cell division, and cell death. The IncuCyte ZOOM imaging system will be located within the Queen’s University Biomedical Imaging Centre, allowing for easy access and effective maintenance of this system. Notably, this imaging platform will provide training for a new generation of cell biologists that will enter the work force with highly desirable skill sets used in industry and academia.

当前的建议支持皇后大学一组新兴细胞生物学研究人员的需求日益增长，以访问旨在积极跟踪活细胞中细胞表型的成像平台。 Incucyte缩放成像系统将为这些研究组提供跟踪细胞分裂，移动，与其他细胞相互作用并经历程序性细胞死亡的工具。这个成像平台将促进从各种组织分离的细胞（心脏，大脑，乳房，胃肠道，外周血）的细胞研究中的新发现。与当前成像系统相比，该仪器中数百个细胞样品的平行研究是一个重大的进步。该系统已开发用于对未标记的细胞以及提供有关活细胞内细胞表型信息的荧光信号的成像。这还将允许女王的研究人员进行高吞吐量筛查和剂量反应研究，以开发新工具以发现细胞运动，细胞 - 细胞相互作用，细胞分裂和细胞死亡的分子基础。 Incucyte缩放成像系统将位于女王大学生物医学成像中心内，可轻松访问和有效维护该系统。值得注意的是，这个成像平台将为新一代的细胞生物学家提供培训，这些新一代生物学家将以行业和学术界使用的高度理想的技能进入劳动力。