A fisherman's new tale: Nutrient-mediated mechanisms of brachial plasticity in rainbow trout.

渔夫的新故事：虹鳟鱼臂可塑性的营养介导机制。

• 批准号: 386197-2013
• 负责人: Vandenberg, Grant
• 金额: $ 2.11万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=630833
• 关键词: fisherman; new; tale; Nutrient; mediated

Phosphorus (P) plays a vital physiological role in intermediary metabolism, and is a key component of DNA/RNA, phospholipids, and skeletal tissues. Despite these key roles, little is known about the physiological effects of P deficiency. There have been a number of studies demonstrating dietary regulation of Pi transport in fish and the regulation of Pi absorption in vivo. In rainbow trout, dietary P restriction increases intestinal Pi uptake due to a number of molecular mechanisms regulating Pi transport. Dietary P restriction has been shown to upregulate both mRNA species as well as to increase Pi uptake by the tissue. Sodium-NaPi is a Pi transporter found in kidney, and is upregulated by dietary P restriction, although recent work has suggested that renal NaPi mRNA is relatively insensitive to changes in dietary P due to the fact that he primary mechanism of renal P regulation is mediated by the trafficking of NaPi protein that is independent of gene transcription. Recent work from my laboratory demonstrates that P-deficient rainbow trout are able to take up P from non feed-derived P. Experiments have consistently revealed that P-deficient fish from surrounding water. We propose a variety of approaches to verify uptake of P from the water by P-deficient fish. We will confirm the uptake of P, and this phenomenon will be characterized, localised using immuno-histological localisation and next generation sequencing techniques/qPCR employed to determine the key pathways that are affected to permit P uptake. The dynamics of the proposed phenomenon and the impact of fish size, P status will be studied. This work will have an important impact on the understanding of the effect of nutrient absorption during nutrient deficiency development, and in particular the adaptive response of freshwater trout to fundamentally alter physiological processes that are currently thought to be insignificant.

磷（P）在中间代谢中起着重要的生理作用，并且是DNA/RNA、磷脂和骨骼组织的关键组分。尽管有这些关键作用，但对缺磷的生理影响知之甚少。已经有许多研究证明了鱼中Pi运输的饮食调节和体内Pi吸收的调节。在虹鳟鱼中，由于许多调节Pi运输的分子机制，饮食P限制增加肠道Pi吸收。膳食磷限制已被证明上调两种mRNA种类，以及增加组织的Pi摄取。钠-NaPi是在肾脏中发现的Pi转运蛋白，并且通过膳食P限制而上调，尽管最近的工作表明肾脏NaPi mRNA对膳食P的变化相对不敏感，这是由于肾脏P调节的主要机制是由NaPi蛋白的运输介导的，其独立于基因转录。我实验室最近的工作表明，缺磷虹鳟鱼能够从非饲料来源的磷中吸收磷。我们提出了各种方法来验证从水中的磷缺乏鱼的吸收。我们将确认P的摄取，并将使用免疫组织学定位和下一代测序技术/qPCR对这种现象进行表征和定位，以确定受影响的关键途径，从而允许P摄取。提出的动态现象和鱼的大小的影响，P状态将进行研究。这项工作将有一个重要的影响，了解营养吸收的影响，在营养缺乏症的发展，特别是适应性反应的淡水鳟鱼从根本上改变生理过程，目前认为是微不足道的。