A novel biofungicide derived from black soldier fly (Hermetia illucens) larval frass**********

消费者越来越多地寻求在尊重越来越严格的环境准则的制度下种植的优质产品。各种来源的有机添加物，如堆肥或粪肥，被认为是良好的营养来源，经常被用作施肥来源。除了与使用有机添加物有关的潜在环境效益外，它们还显示出控制土壤传播的植物病原体的能力。堆肥在抑制植物病害方面的有效性已在文献中描述。考虑到各种蔬菜和观赏植物容易受到由多种病原体引起的真菌病害的影响，使用有机材料的提取物可以防止这些病害造成的损失。我们的研究小组最近的努力开发了利用昆虫幼虫生物转化废弃有机残留物的策略。这种方法将食物废物升级为有价值的动物饲料成分。这种生物过程的第二个产物是幼虫的排泄物，称为“粪便”，它富含有机物质，此外还有被证明对园艺作物有价值的土壤改良剂的宏和微量元素。这种生物资源也是独特微生物群落的丰富来源。我们实验室最近的工作表明，来自我们昆虫幼虫生物转化设施的粪便显著抑制终极腐霉和经济上重要的植物真菌病原体的生长。过滤灭菌的粪便介质导致消除这种影响，表明真菌生长抑制是微生物介导的现象。目前的建议旨在证明昆虫幼虫粪便对园艺作物病害抑制的影响，了解粪便特性对处理植物的增强植物保护作用的影响。我们将采用下一代宏基因组测序来鉴定参与观察到的影响的微生物群落。这项工作将为开发新型生物杀菌剂铺平道路，以帮助生产商追求增强的可持续工业发展。

Consumers are increasingly seeking quality produce grown under systems respecting progressively more restrictive environmental guidelines. Organic amendments of diverse origin such as composts or manure are considered a good source of nutrients and are often used as a source of fertilization. In addition to the potential environmental benefits related to the use of organic amendments, they also have demonstrated an ability to control soil-borne plant pathogens. The effectiveness of composts in the suppression of plant diseases has been described in the literature. Considering that various vegetable and ornamental plants are susceptible to fungal diseases caused by a multitude of pathogens, the use of extracts from organic materials may prevent losses resulting from such diseases.Recent efforts by our research group have developed strategies for biotransforming waste organic residues using insect larvae. This approach upcycles food wastes to produce valuable animal feed ingredients. A secondary product of this bioprocess is larval excrement called 'frass', which is rich in organic matter in addition to maco and micro elements that have proven valuable as soil amendments for horticultural crops. This bioresource is also a rich source of unique microbial communities. Recent work from our lab has demonstrated that frass from our insect larvae bioconversion facility significantly inhibits the growth of Pythium ultimum, and economically-important plant fungal pathogen. Filter-sterilization of the frass media resulted in the elimination of this effect, demonstrating that of fungal growth inhibition is a microbial-mediated phenomenon. The current proposal seeks to demonstrate the impact of insect larval frass on disease suppressiveness in horticultural crops, to understand the impacts of frass characteristics on the augemented phytoprotective effects in treated plants. We will employ next-generation metagenomic sequencing to identify microbial communities involved in the observed effects. This work will pave the way for the development of novel biofungicides to assist producers to pursue enhanced sustainable industry development.