A novel biofungicide derived from black soldier fly (Hermetia illucens) larval frass**********

消费者越来越多地寻求在尊重日益严格的环境指导方针的制度下种植的优质产品。各种来源的有机改良剂，如堆肥或粪便，被认为是一种很好的营养来源，经常被用作肥料来源。除了与使用有机改良剂相关的潜在环境效益外，它们还展示了控制土壤传播的植物病原体的能力。堆肥在抑制植物病害方面的有效性已经在文献中得到了描述。考虑到各种蔬菜和观赏植物容易受到多种病原体引起的真菌病害的影响，使用有机材料提取物可以防止此类疾病造成的损失。最近，我们的研究小组开发了利用昆虫幼虫生物转化废弃有机残留物的策略。这种方法回收食物垃圾来生产有价值的动物饲料成分。这一生物过程的第二个产品是幼虫粪便，名为FRASS，除了被证明作为园艺作物土壤改良剂的Maco和微量元素外，还含有丰富的有机物质。这种生物资源也是独特微生物群落的丰富来源。我们实验室最近的工作表明，我们的昆虫幼虫生物转化设施中的FRAS显著抑制了最终腐霉的生长，这是一种经济上重要的植物真菌病原体。滤膜灭菌消除了这一影响，表明真菌生长抑制是一种微生物介导的现象。目前的建议旨在证明昆虫幼虫FRAS对园艺作物病害抑制的影响，以了解FRASS特性对处理植物增强植物保护作用的影响。我们将使用下一代元基因组测序来识别与观察到的效应有关的微生物群落。这项工作将为开发新型生物杀菌剂铺平道路，以帮助生产商寻求更好的可持续工业发展。

Consumers are increasingly seeking quality produce grown under systems respecting progressively more restrictive environmental guidelines. Organic amendments of diverse origin such as composts or manure are considered a good source of nutrients and are often used as a source of fertilization. In addition to the potential environmental benefits related to the use of organic amendments, they also have demonstrated an ability to control soil-borne plant pathogens. The effectiveness of composts in the suppression of plant diseases has been described in the literature. Considering that various vegetable and ornamental plants are susceptible to fungal diseases caused by a multitude of pathogens, the use of extracts from organic materials may prevent losses resulting from such diseases.Recent efforts by our research group have developed strategies for biotransforming waste organic residues using insect larvae. This approach upcycles food wastes to produce valuable animal feed ingredients. A secondary product of this bioprocess is larval excrement called 'frass', which is rich in organic matter in addition to maco and micro elements that have proven valuable as soil amendments for horticultural crops. This bioresource is also a rich source of unique microbial communities. Recent work from our lab has demonstrated that frass from our insect larvae bioconversion facility significantly inhibits the growth of Pythium ultimum, and economically-important plant fungal pathogen. Filter-sterilization of the frass media resulted in the elimination of this effect, demonstrating that of fungal growth inhibition is a microbial-mediated phenomenon. The current proposal seeks to demonstrate the impact of insect larval frass on disease suppressiveness in horticultural crops, to understand the impacts of frass characteristics on the augemented phytoprotective effects in treated plants. We will employ next-generation metagenomic sequencing to identify microbial communities involved in the observed effects. This work will pave the way for the development of novel biofungicides to assist producers to pursue enhanced sustainable industry development.