Feasibility for 'Real Time' Quantification of Wastewater Bacteriological Composition

从水质和人类健康的角度来看，“真实的时间”病原体检测和量化的概念具有巨大的价值。这不仅从饮用水的角度来看，而且从废水排放的角度来看都是至关重要的。例如，这种方法可以告知公共海滩/娱乐用水，雨水径流排放，下水道溢流质量或抛光池性能仅举几例。TECTA-PDS开发了世界上第一个自动化的，EPA批准的微生物水质测试系统，使其更容易，更快，更便宜地进行现场水质测试E。大肠杆菌到目前为止，这项技术主要集中在确定饮用水的便携性，通过存在-不存在测试大肠杆菌（E。大肠菌群、总大肠菌群和肠球菌。现有技术仅限于废水应用的二进制结果，但TECTA-PDS设备可以提供量化值。这一项目旨在扩大细菌定量范围的细菌特定的废水质量。这将通过（1）确定TECTA是否是一种可行的方法来定量不同来源的废水中的细菌组成;（2）评估现有的校准方法（用于E。大肠菌群、总大肠菌群和肠球菌属）的检测方法;（3）通过自定义校准建立合适的废水检测方案和TECTA参数。在短期内，该项目将在6个月内培训一名硕士和两名本科生HQP。积极的结果将使随后的合作，长期的基础上，涉及实验室规模，然后试点规模的测试与TECTA-PDS合作在比蒂水研究中心在女王大学。快速监测小型、农村和偏远社区细菌废水参数的能力可帮助这些社区以低成本达到其规定的污水排放要求。这反过来又有望改善对目前受废水排放影响的哥伦比亚流域的保护，以满足监管要求。

The concept of a 'real time' pathogen detection and quantification holds tremendous value from a water qualityand human health perspective. This is essential not only from a drinking water perspective, but also from awastewater effluent discharge. For example, such an approach could inform public beach/recreational wateraccess, stormwater runoff discharge, sewer overflow quality or polishing pond performance to name a few.TECTA-PDS developed the world's first automated, EPA-approved microbiological water quality testingsystem, making it easier, faster and less expensive to do on-site water testing for E. coli bacteria. To date, thistechnology has primarily been focused on determining drinking water portability via presence-absence tests forEscherichia coli (E. coli), total coliforms and Enterococcus. The existing technology is limited to binary resultsfor wastewater applications, however TECTA-PDS equipment can provide quantifying values. This projectaims to broaden bacterial quantifiability on a range of bacteria specific to wastewater quality. This will beaccomplised by (1) determining whether TECTA is a viable method of quantifying bacteria composition inwastewater from different sources; (2) assessing whether existing calibrations (for E. coli, total coliforms andEnterococcus) in drinking water are suitable for wastewater; and (3) establishing appropriate wastewater testingprotocols and TECTA parameters via custom calibrations. In the short term, tthe project will train one MAScand two undergraduate HQP over a 6-month period. Positive results will enable subsequent collaborations, on alonger term basis, involving bench-scale, then pilot-scale testing in collaboration with TECTA-PDS at theBeaty Water Research Centre at Queen's University. The capacity for rapid monitoring of bacterial wastewaterparameters for small, rural and remote communities could assist these communities to achieve their regulatoryeffluent discharge requirements at a low cost. This in turn is expected to improve protection for Canadianwatersheds which are currently being impacted by wastewater discharge in exceedance of regulatoryrequirements.