Investigation and development of an accurate ethanol content measurement method for kombucha

布赫公司生产有机工艺康普茶，并将其产品分销给安大略省200多家零售商。Booch Inc.经营了两年多，是安大略最大的康普茶啤酒厂，加拿大第二大，并被公认为这个不断发展的行业的领导者和潮流引领者。康普茶是一种天然的碳酸饮料，由红茶或绿色茶和糖发酵制成，含有各种细菌和酵母菌。目前，市场上还没有准确的方法来测量红茶菌酿造过程中的酒精含量。一些啤酒制造商甚至由于其产品中酒精含量的不准确报告而经历了法律的影响。Booch已经确定了一种潜在的新产品，红茶菌乙醇含量测量套件，它将包括一份详细的协议，概述了一种经过验证的方法，用于测量红茶菌酿造时的乙醇含量。为了探索这种潜在的新产品，Booch将与Fanshawe学院的应用科学和技术学院合作。学生研究助理KarenBuchholz将与主要研究者合作，开发并比较三种测量酿造红茶菌中乙醇含量的方法。根据测量方法的发展，学生将研究哪种方法最有效，并准备一份详细说明所选方法的协议。该应用研究项目的结果将有助于Booch将其客户市场扩展到其他康普茶酿造商。它帮助Booch和其他红茶菌酿造商做出决策，例如是否可以改变发酵条件以生产可接受的产品，或者是否应该放弃一批以节省时间和资源。此外，它将确保所有年龄段的康普茶产品都安全，并且该饮料被归类为非酒精饮料（低于0.05%）。3表格103（2011 W），第3页，共7页第二

Booch Inc. manufactures organic craft kombucha and distributes their product to over 200 retailers acrossOntario. Operating for just over 2 years, Booch Inc. is the largest kombucha brewery in Ontario, second largestin Canada, and recognized as a leader and trendsetter within this growing industry. Kombucha is a naturallycarbonated beverage made by fermenting black or green tea and sugar with a culture of various bacteria andyeasts. Currently, there is no accurate method available on the market to measure alcohol content during thekombucha brewing process. Some brewers have even experienced legal repercussions due to inaccuratereporting of alcohol content in their product. Booch has identified a potential new product, a kombucha ethanolcontent measurement kit, which would consist of a detailed protocol outlining a proven method for measuringethanol content while kombucha is brewing. To explore this potential new product Booch will be collaboratingwith Fanshawe College's Applied Science and Technology School. Working with Principal Investigator, KarenBuchholz, a student research assistant will develop and compare three methods of measuring the ethanolcontent in brewing kombucha. Following development of the measurement methods, the student willinvestigate which method is most effective, and prepare a protocol detailing the selected method. The results ofthis applied research project will assist Booch in expanding their client market to other kombucha brewers. Itwill help Booch and other kombucha brewers in making decisions such as whether or not fermentationconditions can be altered to produce an acceptable product, or if a batch should be abandoned to save time andresources. Additionally, it will ensure safe kombucha products for all ages, and that the beverage is classifiedas non-alcoholic (less than 0.05%).3Form 103 (2011 W), page 3 of 7Second