Super Resolution Core Facility Upgrade

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Super resolution microscopy has broken the optical diffraction barrier enabling unprecedented imaging of <100 nm cellular and molecular structures. Resolution of single-molecule localization microscopy (SMLM), based on the repeated activation (blinking) of small numbers of discrete fluorophores, approaches 10 nm. Stimulated emission depletion (STED) super resolution microscopy, with a resolution of 40-50 nm in XY, is more versatile than SMLM and can image multiple fluorophores and live cells. At UBC’s Life Sciences Institute (LSI), we have developed improved hardware (real-time drift control) and analytical approaches (machine learning network analysis and graph theory) for SMLM as well as 3-channel, 3D analysis using STED. Upgrading our capability with SMLM and STED is now critical.