Super Resolution Core Facility Upgrade
超分辨率核心设施升级
基本信息
- 批准号:RTI-2018-00767
- 负责人:
- 金额:$ 10.74万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Research Tools and Instruments
- 财政年份:2017
- 资助国家:加拿大
- 起止时间:2017-01-01 至 2018-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Super resolution microscopy has broken the optical diffraction barrier enabling unprecedented imaging of <100 nm cellular and molecular structures. Resolution of single-molecule localization microscopy (SMLM), based on the repeated activation (blinking) of small numbers of discrete fluorophores, approaches 10 nm. Stimulated emission depletion (STED) super resolution microscopy, with a resolution of 40-50 nm in XY, is more versatile than SMLM and can image multiple fluorophores and live cells. At UBC’s Life Sciences Institute (LSI), we have developed improved hardware (real-time drift control) and analytical approaches (machine learning network analysis and graph theory) for SMLM as well as 3-channel, 3D analysis using STED. Upgrading our capability with SMLM and STED is now critical.
超分辨率显微镜打破了光学衍射障碍,使<100纳米的细胞和分子结构成像成为可能。基于少量离散荧光团的重复激活(闪烁),单分子定位显微镜(SMLM)的分辨率接近10 nm。受激发射损耗(STED)超分辨率显微镜,在XY分辨率为40-50纳米,比SMLM更通用,可以成像多个荧光团和活细胞。在UBC生命科学研究所(LSI),我们为SMLM开发了改进的硬件(实时漂移控制)和分析方法(机器学习网络分析和图论),以及使用STED进行3通道3D分析。现在,通过SMLM和STED来提升我们的能力至关重要。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Nabi, Ivan的其他文献
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{{ truncateString('Nabi, Ivan', 18)}}的其他基金
Nanodomain Structure of the Endoplasmic Reticulum by Super Resolution Microscopy
通过超分辨率显微镜观察内质网的纳米域结构
- 批准号:
RGPIN-2019-05179 - 财政年份:2022
- 资助金额:
$ 10.74万 - 项目类别:
Discovery Grants Program - Individual
tauSTED: Fluorescent Lifetime Upgrade for Stimulated Emission Depletion Super-Resolution Microscope
tauSTED:受激发射损耗超分辨率显微镜的荧光寿命升级
- 批准号:
RTI-2022-00465 - 财政年份:2021
- 资助金额:
$ 10.74万 - 项目类别:
Research Tools and Instruments
Nanodomain Structure of the Endoplasmic Reticulum by Super Resolution Microscopy
通过超分辨率显微镜观察内质网的纳米域结构
- 批准号:
RGPIN-2019-05179 - 财政年份:2021
- 资助金额:
$ 10.74万 - 项目类别:
Discovery Grants Program - Individual
Super-resolution microscopy network analysis: Drug target validation for cystic fibrosis
超分辨率显微镜网络分析:囊性纤维化的药物靶点验证
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538851-2019 - 财政年份:2020
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$ 10.74万 - 项目类别:
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Artificial intelligence-based imaging platform for COVID-19 infection of organoids
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553515-2020 - 财政年份:2020
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Nanodomain Structure of the Endoplasmic Reticulum by Super Resolution Microscopy
通过超分辨率显微镜观察内质网的纳米域结构
- 批准号:
RGPIN-2019-05179 - 财政年份:2020
- 资助金额:
$ 10.74万 - 项目类别:
Discovery Grants Program - Individual
Nanodomain Structure of the Endoplasmic Reticulum by Super Resolution Microscopy
通过超分辨率显微镜观察内质网的纳米域结构
- 批准号:
RGPIN-2019-05179 - 财政年份:2019
- 资助金额:
$ 10.74万 - 项目类别:
Discovery Grants Program - Individual
Super-resolution microscopy network analysis: Drug target validation for cystic fibrosis
超分辨率显微镜网络分析:囊性纤维化的药物靶点验证
- 批准号:
538851-2019 - 财政年份:2019
- 资助金额:
$ 10.74万 - 项目类别:
Collaborative Health Research Projects
Glycan regulation of cell-cell junction dynamics and architecture
细胞-细胞连接动力学和结构的聚糖调节
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227925-2013 - 财政年份:2018
- 资助金额:
$ 10.74万 - 项目类别:
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Market research study proposal for an assessment of market opportunities for application of 3D pattern analysis to single-molecule localization microscopy (SMLM)
评估将 3D 模式分析应用于单分子定位显微镜 (SMLM) 的市场机会的市场研究建议
- 批准号:
514313-2017 - 财政年份:2017
- 资助金额:
$ 10.74万 - 项目类别:
Idea to Innovation
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