Super Resolution Core Facility Upgrade

超分辨率核心设施升级

• 批准号: RTI-2018-00767
• 负责人: Nabi, Ivan
• 金额: $ 10.74万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642159
• 关键词: Super; Resolution; Core; Facility; Upgrade

Super resolution microscopy has broken the optical diffraction barrier enabling unprecedented imaging of <100 nm cellular and molecular structures. Resolution of single-molecule localization microscopy (SMLM), based on the repeated activation (blinking) of small numbers of discrete fluorophores, approaches 10 nm. Stimulated emission depletion (STED) super resolution microscopy, with a resolution of 40-50 nm in XY, is more versatile than SMLM and can image multiple fluorophores and live cells. At UBC’s Life Sciences Institute (LSI), we have developed improved hardware (real-time drift control) and analytical approaches (machine learning network analysis and graph theory) for SMLM as well as 3-channel, 3D analysis using STED. Upgrading our capability with SMLM and STED is now critical.

超分辨率显微镜打破了光学衍射障碍，使<100 nm的细胞和分子结构前所未有的成像。基于少量离散荧光团的重复激活（闪烁），单分子定位显微镜（SMLM）的分辨率接近10 nm。受激发射耗尽（STED）超分辨率显微镜，XY分辨率为40-50 nm，比SMLM更通用，可以对多个荧光团和活细胞进行成像。在UBC生命科学研究所（LSI），我们已经开发了改进的硬件（实时漂移控制）和分析方法（机器学习网络分析和图论），用于SMLM以及使用STED的3通道3D分析。现在，使用SMLM和STED升级我们的能力至关重要。