The biomineralization of metals by microorganisms in organic sediments

有机沉积物中微生物对金属的生物矿化

• 批准号: RGPIN-2014-04064
• 负责人: Glasauer, Susan
• 金额: $ 2.7万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=651993
• 关键词: biomineralization; metals; microorganisms; organic; sediments

Microorganisms drive key processes controlling the mobility of metals that are contained in minerals, adsorbed to organic and inorganic substrates, and dissolved in water at the Earth's surface. My research program centers on explaining how microbes common in Earth surface environments impact metal mobility by controlling electron transfer reactions through respiration; some microbes can use metals as electron donors and terminal electron acceptors. Changes to the oxidation state of some metals as a consequence of respiration can drastically impact their solubility. Biominerals result when such processes result in metal-rich precipitates. It is unknown how the microbial respiration of metals contributes to metal cycling in cold, northern peat wetlands. This is an important knowledge gap applicable to all peatlands, but I have chosen to focus on areas impacted by mining activities because: 1) the elevated concentrations of target elements provide a unique setting to understand metal biogeochemistry; 2) the capacity of wetlands to buffer metal releases to water systems is poorly understood; 3) the environmental impacts of some chemical discharges can be devastating to ecosystem health. Future climate warming and more extreme fluctuations in hydric regimes can be expected to affect microbial processes by altering microbial respiration, and by impacting the solubility of biominerals. This research is targeted at understanding a) the impacts of redox fluctuations induced by microbial respiration and oxygen exposure on the interactions between organic matter, iron and important target elements associated with some mines in northern Canada (U, Cr); b) the critical factors controlled by microbes and related to climate that impact biomineralization of target metals in peat substrates, and c) the capacity of native peat wetland systems to buffer metal fluxes during climate fluctuations.**Addressing these objectives requires an interdisciplinary approach grounded in both geochemistry and microbiology. Projects to achieve the objectives include: *1) The dynamic relationship between iron oxide, organic matter, and U or Cr during cycles of reducing and oxidizing conditions created by microbial respiration, using batch systems; *2) Biomineralization of U, Cr, and Fe in a peat wetland substrate using flow cells, during changes in temperature and water saturation; *3) Changes in microbial community structure during simulated climate fluctuations in the flow cell system, in the presence of U, Cr and Fe; *4) The links between metal budgets in native, in situ peatlands during wet, cold conditions (spring) and warm, dry conditions (early fall); *5) Seasonal impacts on microbial communities and the correlation of specific microbial groups with metal dynamics in native peatlands.**The research program will provide important training in laboratory and field techniques for graduate and undergraduate students. Students will be trained to use a multidisciplinary range of approaches that encompass observational and analytical scales that range from molecular to macroscopic. Developing novel experimental methods, such as X-ray absorption spectroscopy and analytical metal chemistry techniques, will provide important training, and will contribute critically to meeting the objectives of my research program. The outcome will be new fundamental knowledge on the dynamics of metal-microbe interactions in organic wetlands.

微生物驱动着控制金属流动性的关键过程，这些金属包含在矿物中，吸附在有机和无机基质上，并溶解在地球表面的水中。我的研究计划集中在解释地球表面环境中常见的微生物如何通过呼吸控制电子转移反应来影响金属的流动性；一些微生物可以利用金属作为电子供体和终端电子受体。由于呼吸作用，某些金属的氧化状态发生变化，这会极大地影响它们的溶解度。当这样的过程导致富金属沉淀时，就会产生生物矿物。目前尚不清楚在寒冷的北部泥炭湿地中，金属的微生物呼吸如何促进金属循环。这是适用于所有泥炭地的重要知识差距，但我选择重点关注受采矿活动影响的地区，因为：1)目标元素浓度升高为了解金属生物地球化学提供了独特的环境；2)湿地缓冲向水系统释放金属的能力知之甚少；3)一些化学物质排放的环境影响可能对生态系统健康造成毁灭性影响。未来气候变暖和氢气制度的更极端波动预计会通过改变微生物呼吸作用和影响生物矿物质的溶解度来影响微生物过程。这项研究的目标是了解a)微生物呼吸和氧气暴露引起的氧化还原波动对有机质、铁和与加拿大北部一些矿山有关的重要目标元素(铀、铬)之间相互作用的影响；b)受微生物控制并与气候有关的影响泥炭基质中目标金属生物矿化的关键因素；以及c)原生泥炭湿地系统在气候波动期间缓冲金属通量的能力。**解决这些目标需要以地球化学和微生物学为基础的跨学科方法。实现这些目标的项目包括：*1)利用间歇系统，在微生物呼吸所创造的还原和氧化条件的循环中，氧化铁、有机物和U或Cr之间的动态关系；*2)在温度和水分饱和变化期间，利用流动细胞对泥炭湿地基质中的U、Cr和Fe进行生物矿化；*3)在流动细胞系统中模拟气候波动期间微生物群落结构的变化；*4)在潮湿、寒冷条件(春季)和温暖、干燥条件(初秋)期间，天然原地泥炭地金属收支之间的联系；*5)对微生物群落的季节性影响以及特定微生物群与原生泥炭地金属动力学的相关性。**该研究计划将为研究生和本科生提供重要的实验室和野外技术培训。学生将被训练使用多学科的方法，包括从分子到宏观的观察和分析尺度。开发新的实验方法，如X射线吸收光谱和分析金属化学技术，将提供重要的培训，并将对实现我的研究计划的目标做出关键贡献。其成果将是关于有机湿地中金属-微生物相互作用动力学的新的基础知识。