Detection of tenacibaculum sp. the cause of mouthrot in Atlantic salmon raised by Marine Harvest Canada****

加拿大海洋丰收公司在不列颠哥伦比亚省可持续地饲养大西洋鲑鱼。网纹夜蛾是一种重要的网纹夜蛾生产病害，由网纹夜蛾引起。损失是由于死亡造成的，但同样重要的是由于增长放缓。受影响的鱼的治疗费用很高，而且经常不得不重复，从而产生巨大的成本。与这种疾病相关的细菌也可能对现有的抗菌剂产生抗药性。因此，除抗菌治疗之外的管理策略是非常必要的。这项提议将调整和创建定量聚合酶链式反应(QPCR)分析，以量化负责的细菌。了解鱼、环境和水中有机体的藏身之处，以及了解暴发前、暴发期间和暴发后何时出现高细菌载量，是更好地管理暴发所需的关键信息。这些检测还可以用来评估抗菌治疗的效果，并了解为什么它们经常不成功。这些检测将改进MHC网络点的鼠喉主动管理，并应允许除抗菌治疗外的其他选择，或减少抗菌药物的使用需求。**

Marine Harvest Canada sustainably raises Atlantic salmon in British Columbia. An important production disease affecting smolts in netpens is mouthrot caused by Tenacibaculum sp. Losses occur due to mortality but as importantly due to reduced growth. Treatment of affected fish is expensive and often has to be repeated incurring significant costs. The bacteria associated with this disease can also be resistant to the available antimicrobial agents. Management strategies other than antimicrobial therapy are therefore of critical need. This proposal will adapt and create quantitative polymerase chain reaction (qPCR) assays for quantification of the responsible bacteria. Understanding where the organism is harboured in the fish, environment and water and knowing when high bacterial loads are present before, during and after an outbreak are key information needed to better manage outbreaks. The assays can also be used to evaluate the efficacy of antimicrobial treatments and understand why they are often not successful. These assays will allow improvements to proactive management of mouthrot at MHC netpen sites and should allow options other than antimicrobial therapy or that reduce the need for antimicrobial use. **