Detection of tenacibaculum sp. the cause of mouthrot in Atlantic salmon raised by Marine Harvest Canada****

检测粘杆菌属。

• 批准号: 533726-2018
• 负责人: Lumsden, John
• 金额: $ 1.82万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=658093
• 关键词: Detection; tenacibaculum; sp.; cause; mouthrot

Marine Harvest Canada sustainably raises Atlantic salmon in British Columbia. An important production disease affecting smolts in netpens is mouthrot caused by Tenacibaculum sp. Losses occur due to mortality but as importantly due to reduced growth. Treatment of affected fish is expensive and often has to be repeated incurring significant costs. The bacteria associated with this disease can also be resistant to the available antimicrobial agents. Management strategies other than antimicrobial therapy are therefore of critical need. This proposal will adapt and create quantitative polymerase chain reaction (qPCR) assays for quantification of the responsible bacteria. Understanding where the organism is harboured in the fish, environment and water and knowing when high bacterial loads are present before, during and after an outbreak are key information needed to better manage outbreaks. The assays can also be used to evaluate the efficacy of antimicrobial treatments and understand why they are often not successful. These assays will allow improvements to proactive management of mouthrot at MHC netpen sites and should allow options other than antimicrobial therapy or that reduce the need for antimicrobial use. **

Marine Harvest Canada在不列颠哥伦比亚省可持续地养殖大西洋鲑鱼。一个重要的生产疾病，影响小鲑鱼在网围栏是mouthrot所造成的Tenacibaculum sp.损失发生由于死亡率，但同样重要的是由于减少增长。对受影响鱼类的治疗费用昂贵，而且往往不得不反复进行，从而产生巨大的费用。与这种疾病相关的细菌也可以对现有的抗菌剂产生耐药性。因此，除抗菌治疗外的其他管理策略至关重要。该提案将调整和创建定量聚合酶链反应（qPCR）测定法，用于定量责任细菌。了解生物体在鱼类、环境和水中的藏身之处，并了解在爆发之前、期间和之后何时存在高细菌负荷，是更好地管理爆发所需的关键信息。该检测也可用于评估抗菌治疗的有效性，并了解为什么它们通常不成功。这些检测将允许改进在MHC网围栏站点的mouthrot的主动管理，并且应该允许除抗菌治疗以外的选择，或者减少对抗菌剂使用的需要。 **