Surveillance for Tenacibaculum sp. the cause of mouth rot in Atlantic salmon raised by MOWI West Canada

加拿大西部渔业部在不列颠哥伦比亚省可持续地养殖大西洋鲑鱼。影响幼崽的一个重要生产疾病是由腱杆菌引起的口喉病。损失主要是由于死亡，但同样重要的是由于生长减少。治疗受影响的鱼是昂贵的，而且经常需要重复治疗，从而产生巨大的成本。与这种疾病相关的细菌也可能对现有的抗生素具有耐药性。因此，除抗菌药物治疗外的其他管理策略是至关重要的。该提案将使用根据NSERCEngage提案开发的定量聚合酶链反应（qPCR）测定来定量负责的细菌。了解微生物在鱼类、环境和水中的藏身之处，以及在疫情爆发之前、期间和之后何时出现高细菌负荷，是更好地管理疫情所需的关键信息。这些检测也可用于评估抗菌治疗的疗效，并了解它们通常不成功的原因。这些检测将有助于改善MHC监测点的口腔主动管理，并应允许除抗菌治疗外的其他选择或减少抗菌使用的需要。

MOWI West Canada sustainably raises Atlantic salmon in British Columbia. An important productiondisease affecting smolts in netpens is mouthrot caused by Tenacibaculum sp. Losses occur due to mortality butas importantly due to reduced growth. Treatment of affected fish is expensive and often has to be repeatedincurring significant costs. The bacteria associated with this disease can also be resistant to the availableantimicrobial agents. Management strategies other than antimicrobial therapy are therefore of critical need.This proposal will use quantitative polymerase chain reaction (qPCR) assays developed under a NSERCEngage proposal for quantification of the responsible bacteria. Understanding where the organism is harbouredin the fish, environment and water and knowing when high bacterial loads are present before, during and afteran outbreak are key pieces of information needed to better manage outbreaks. The assays can also be used toevaluate the efficacy of antimicrobial treatments and understand why they are often not successful. Theseassays will allow improvements to proactive management of mouthrot at MHC netpen sites and should allowoptions other than antimicrobial therapy or that reduce the need for antimicrobial use.