Temperature-sensitive proteases for research and diagnostics

用于研究和诊断的温度敏感蛋白酶

• 批准号: 523350-2018
• 负责人: Nano, Francis
• 金额: $ 9.07万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=660631
• 关键词: Temperature; sensitive; proteases; research; diagnostics

The objective of this proposal is to develop a family of temperature-sensitive proteases that can be used in molecular biology research and in automated diagnostic systems to detect human diseases. A protease is an enzyme that attacks and degrades other proteins. Often in the research or diagnostic laboratory a series of steps are used, and each step uses a different enzyme, which is a protein. In these situations a major challenge is to remove the enzyme from one step before moving on to the next step. A temperature-sensitive protease addresses this problem by providing a way of removing an enzyme through degradation, and then removing the protease activity through mild heating. Currently, there are no temperature-sensitive proteases commercially available, thus our discovery will fill a currently unmet need.

本提案的目标是开发一个温度敏感的蛋白酶家族，可用于分子生物学研究和自动诊断系统，以检测人类疾病。蛋白酶是一种攻击和降解其他蛋白质的酶。通常在研究或诊断实验室中使用一系列步骤，每个步骤使用不同的酶，这是一种蛋白质。在这些情况下，一个主要的挑战是在进入下一步之前从一步中去除酶。温度敏感型蛋白酶解决了这个问题，它提供了一种通过降解去除酶的方法，然后通过温和的加热去除蛋白酶的活性。目前，还没有商业上可用的温度敏感蛋白酶，因此我们的发现将填补目前未满足的需求。