Mapping disease resistance, quality and yield traits in cannabis sativa L.

绘制大麻的抗病性、品质和产量性状。

• 批准号: 530255-2018
• 负责人: Pauls, Karl
• 金额: $ 16.81万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=661758
• 关键词: Mapping; disease; resistance; quality; yield

Cannabis is an ancient source of fibre, oil and medicines. This plant is largely unexplored due to its drug content and laws against production, trafficking and consumption. However, with the advent of laws to regulate its production and legal medical and recreation uses more information about its genetic diversity and the genetic relationships among clones is required. This will be especially important for designing plant breeding strategies for disease resistant varieties in the future. The recent recognition of cannabis as an important medicinal plant has resulted in some research on different functional and evolutionary aspects of this plant, including a draft genome and transcriptome. However, there is little published information on quantitative trait loci (QTL) associated with economically important traits. Genetic characterization of several important traits, including: female flower bud yield, THC and CBD content and disease resistance are required for effective selection and breeding economically viable cannabis varieties. *The objectives of the proposed collaborative project between the University of Guelph and Canopy Growth Corporation (Tweed) are to:**- better understand the genetic variation among important commercial strains at Canopy Growth Corp**- develop a genetic mapping population between a disease resistant cannabis line and other lines with desirable production traits (such as bud yield, cannabanoid cannabinoid content, plant architecture, plant maturity)**- identify SNPs among the parents and the lines in the population lines by deep and skim sequencing,**- derive a linkage map for cannabis based on segregation of the markers in the population, and**- map quantitative trait loci (QTL) for important production traits in cannabis, and**- develop molecular markers for economically important traits.*This information will be useful to develop molecular methods for selecting powdery mildew resistant cannabis strains and implementing these methods in breeding novel cannabis varieties.

大麻是纤维、油和药物的古老来源。由于其药物含量和禁止生产、贩运和消费的法律，这种植物基本上未被开发。然而，随着法律的出现，以规范其生产和法律的医疗和娱乐用途更多的信息，其遗传多样性和克隆之间的遗传关系是必要的。这对于未来设计抗病品种的植物育种策略尤为重要。最近认识到大麻是一种重要的药用植物，导致对这种植物的不同功能和进化方面进行了一些研究，包括基因组草图和转录组。然而，与经济重要性状相关的数量性状基因座（QTL）的研究却很少。为了有效地选择和培育经济上可行的大麻品种，需要对包括雌花蕾产量、四氢大麻酚和CBD含量以及抗病性在内的几个重要性状进行遗传鉴定。* 圭尔夫大学和Canopy Growth Corporation（Tweed）之间拟议合作项目的目标是：**-更好地了解Canopy Growth Corp重要商业品系之间的遗传变异 **-在抗病大麻品系与具有理想生产性状的其他品系之间建立遗传图谱（如芽产量、大麻素含量、植物结构、植物成熟度）**-通过深度和脱脂测序鉴定亲本和群体品系中的品系之间的SNP，**-基于群体中标记的分离导出大麻的连锁图，** 绘制大麻重要生产性状的数量性状基因座（QTL），** 开发重要经济性状的分子标记。这些信息将有助于开发分子方法来选择抗白粉病的大麻菌株，并在培育新的大麻品种中实施这些方法。