Mechanoculture FX Optimization of Long Term 3D Cell Culture and Quantification

长期 3D 细胞培养和定量的机械培养 FX 优化

• 批准号: 530791-2018
• 负责人: Pelling, Andrew
• 金额: $ 1.82万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=661878
• 关键词: Mechanoculture; FX; Optimization; Long; Term

Living cells in the body are exposed to mechanical forces that provide critical signals to regulate cell fate and**function. Understanding how mechanical forces regulate cell function is therefore broadly important to our**understanding of cell and tissue dysfunction in disease. Importantly, the design of medical devices,**biomaterials, and engineered tissues for repair or replacement is informed by our understanding of how living**cells sense and respond to mechanical forces. The problem faced by biomedical researchers is that traditional**cell culture substrate materials do not appropriately mimic the mechanical cues that arise in the in vivo**microenvironment because they are rigid and static. To advance discovery and innovation in mechanobiology,**CellScale Biomaterials Testing Inc. has developed the MechanoCulture FX system, a novel culture technology**for cell stretching experimentation. In this project, CellScale will collaborate with researchers to develop novel**cell culture plate designs which will allow for long-term cell culture, microscopy and quantification in order to**advance our understanding of mechanobiology. These new innovations are expected to establish a new**standard in commercial cell stretching systems and have rapid impact. CellScale is a leader in providing such**tools and innovations to bioscience and regenerative medicine researchers around the globe.

体内的活细胞受到机械力的作用，这些机械力提供了调节细胞命运和功能的关键信号。因此，了解机械力如何调节细胞功能对于我们理解疾病中的细胞和组织功能障碍非常重要。重要的是，医疗器械、生物材料和用于修复或替换的工程组织的设计都是基于我们对活细胞如何感知和响应机械力的理解。生物医学研究人员面临的问题是，传统的细胞培养基质材料不能适当地模拟体内微环境中出现的机械提示，因为它们是刚性和静态的。为了推进机械生物学的发现和创新，**CellScale Biomaterials Testing Inc.已经开发了机械培养FX系统，这是一种用于细胞拉伸实验的新型培养技术。在这个项目中，CellScale将与研究人员合作开发新的细胞培养板设计，这将允许长期的细胞培养，显微镜和定量，以促进我们对机械生物学的理解。这些新的创新有望在商业细胞拉伸系统中建立新的标准，并产生快速的影响。CellScale是为地球仪的生物科学和再生医学研究人员提供此类工具和创新的领导者。