Osmotic induced changes in cell volume: a regulator of protein metabolism in skeletal muscle.

渗透引起的细胞体积变化：骨骼肌蛋白质代谢的调节剂。

• 批准号: 261427-2012
• 负责人: Roy, Brian
• 金额: $ 1.82万
• 依托单位: Brock University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=663814
• 关键词: Osmotic; induced; changes; cell; volume

The long-term goal of this research is to understand how fluid shifts both into and out of muscle tissue impacts the regulation of protein metabolism in this tissue. We also plan to develop effective strategies to exploit such changes within this tissue, as muscle is the largest fluid reservoir in the body. Over the short term the experiments will establish a timeline of the influence of fluid shifts on protein metabolism in muscle. Furthermore we will also determine if there is an interaction between fluid shifts and muscle contraction (exercise) induced changes in protein metabolism. We will also look at how muscle takes up amino acids (building blocks of proteins) to see if these processes are changed with these fluid shifts. Finally, we will also determine if such fluid shifts can counter or lessen the effect of drugs that cause muscle wasting. Overall, this research will contribute to a greater understanding of the role of fluid shifts in the regulation of protein metabolism. Thus, findings from this research will provide biological and evidence-based rationale for developing fluid-based strategies to manipulate skeletal muscle protein metabolism in circumstances where fluid balance is altered. Furthermore, this work will contribute to the development of pharmaceutical strategies and products that could reduce muscle wasting.

这项研究的长期目标是了解液体如何流入和流出肌肉组织，影响该组织中蛋白质代谢的调节。 我们还计划开发有效的策略来利用这种组织内的这种变化，因为肌肉是体内最大的液体储存库。 在短期内，实验将建立一个时间轴的流体转移对肌肉蛋白质代谢的影响。 此外，我们还将确定液体转移和肌肉收缩（运动）诱导的蛋白质代谢变化之间是否存在相互作用。 我们还将研究肌肉是如何吸收氨基酸（蛋白质的组成部分）的，看看这些过程是否会随着这些液体的变化而改变。 最后，我们还将确定这种液体转移是否可以抵消或减轻导致肌肉萎缩的药物的作用。 总的来说，这项研究将有助于更好地了解液体转移在蛋白质代谢调节中的作用。因此，这项研究的结果将为开发基于液体的策略提供生物学和循证依据，以在液体平衡改变的情况下操纵骨骼肌蛋白质代谢。 此外，这项工作将有助于开发可以减少肌肉萎缩的药物策略和产品。