Dairy bioactive nutrients synergistic effects against inflammation: nutrigenomics and metabolomics approaches

目前的证据支持乳制品生物活性营养素对炎症反应的益处。虽然这些作用的具体机制尚未完全阐明，但令人信服的证据支持这一假设，即乳制品成分，如脂肪和蛋白质，积极影响促炎分子的释放。此外，早期的机制研究已经调查了个别乳制品脂肪和蛋白质对炎症的有益作用。然而，这些生物活性分子协同作用的可能性从未被研究过。拟议研究计划的长期目标是表征新型生物活性营养素对哺乳动物炎症的联合作用，并确定所需的食物富集。下一个五年目标是确定特别存在于乳制品脂肪和蛋白质/氨基酸中的常量营养素成分是否可以单独，叠加或协同调节炎症反应。我们将使用基因组学和代谢组学技术来识别分子特征和乳制品成分对培养细胞中炎症指标的作用机制。根据我们的试点工作，这项研究计划可能会导致高度保护性的脂肪/蛋白质组合的鉴定，我们预计未来的方向将涉及体内验证步骤。总之，这些体外研究将有助于更好地了解乳制品生物活性分子对炎症反应的作用机制。靶向抑制相关组织中的各种促炎细胞因子代表了用于动物和人类摄入的补充剂或功能性成分的令人兴奋的新机会。此外，使用创新的OMIC技术将增加我们对生物活性分子的有益分子效应的了解。总之，NSERC计划将使我们能够将具有生物活性成分最佳组合的创新和有竞争力的产品推向市场。这样的研究计划将促进该国营养基因组学和食品科学领域研究的发展和扩大，并将加强对HQP的培训，这些HQP将继续对加拿大经济和以加拿大为基础的研究和发现产生积极影响。

Current evidences support a benefit for dairy bioactive nutrients against inflammatory reactions. Although the specific mechanisms underlying these effects have not been fully elucidated, convincing evidences support the hypothesis that dairy components, such as fat and proteins, actively impact the release of pro-inflammatory molecules. Further, earlier mechanistic studies have investigated the beneficial effects of individual dairy fat and proteins on inflammation. However, the possibility that these bioactive molecules synergise together has never been studied. The long-term objective of the proposed research program is to characterize the combined actions of novel bioactive nutrients on inflammation in mammals and to define the required food enrichment. The next five-year objective is to determine whether macronutrient components, present specifically in dairy fats and proteins/amino acids, can individually, additively or synergistically, regulate inflammatory reactions. We will use genomics and metabolomics technologies to identify the molecular signatures and the mechanism of action of components of dairy products on inflammation indicators in cultured cells. Based on our pilot work, this research initiative may lead to the identification of highly protective fat/protein combination(s), and we anticipate that future directions will involve in vivo validations steps. Together, these in vitro studies will help to better understand the mechanisms of action of dairy bioactive molecules on inflammatory reactions. The targeted suppression of various pro-inflammatory cytokines in relevant tissues represents an exciting new opportunity for supplements or functional ingredients for animal and human intake. Further, the use of innovative OMICs technologies will increase our knowledge on the beneficial molecular effects of bioactive molecules. Taken together, this NSERC program will allow us to bring innovative and competitive products with optimal combination of bioactive components to the marketplace. Such a research program will promote the development and expansion of research in the field of nutrigenomics and food science in the country and will enhance the training of HQPs who will go on to positively impact the Canadian economy and Canadian-based research and discovery.