Multifactorial regulation of reproduction and gonadal development in fish

鱼类繁殖和性腺发育的多因素调控

• 批准号: RGPIN-2017-04849
• 负责人: Habibi, Hamid
• 金额: $ 2.84万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=687390
• 关键词: Multifactorial; regulation; reproduction; gonadal; development

My research objective over many years has been to understand hormonal control of reproduction and growth in fish by studying brain regulation of gonadotropin and growth hormone production. We have contributed to the understanding of mechanisms by which hypothalamic gonadotropin-releasing hormone regulates pituitary luteinizing hormone, follicle stimulating hormone and growth hormone production. We have also provided novel information on paracrine regulation of testicular and ovarian function in fish by investigating the local effects of gonadal hormones in the regulation of gametogenesis (egg and sperm production) and steroidogenesis. Our recent findings suggest that gonadotropin-inhibitory hormone (GnIH) and thyroid hormones are among the key factors that influence the integrated control of reproduction and growth in fish. The proposed study will build on our past work and focus on three primary research areas conducted simultaneously in my group: (1) The role of thyroid hormones and GnIH, working in concert with brain neuro-hormones and gonadal hormones, to regulate reciprocal control of pituitary gonadotropins and growth hormone production; (2) The role of thyroid hormones and GnIH, working in concert with pituitary and gonadal hormones, to regulate ovarian and testicular development and differentiation. (3) Changes in metabolic pathways required to energetically support and sustain prolonged periods of reproduction and growth. The proposed studies in these areas will be designed to meet the following major research objectives:*** ***1. Understand the integrated control of gonadotropin and growth hormone production***2. Understand the integrated regulation of testicular and ovarian development and function during reproductive and growth phases***3. Elucidate the regulation of metabolism to sustain reproduction and growth******We will use state-of-the-art facilities and innovative experimental approaches to investigate the proposed objectives. These projects will be used as a vehicle for the training of HQP in the broad areas of comparative endocrinology, and will involve three PhD and two MSc students as well as several undergraduate students, visiting students and scientists. The proposed research will enhance our understanding of the integrated reciprocal control of reproduction and growth and associated changes in metabolism in fish, as well as providing important information on integrated regulation of testicular and ovarian development and function. The results will be relevant to all vertebrate species by providing an insight into the mechanisms by which delicate balance between reproduction and growth is regulated. Furthermore, the proposed studies will have translational potential in aquaculture and environmental toxicology by improving our ability to assess adverse impacts of environmental contaminants with hormone-like activities in the aquatic ecosystem.

多年来，我的研究目标一直是通过研究促性腺激素和生长激素的大脑调节来了解激素对鱼类繁殖和生长的控制。我们对下丘脑促性腺激素释放激素调节垂体黄体生成素、卵泡刺激素和生长激素产生的机制做出了贡献。我们还通过研究性腺激素在配子发生(卵子和精子产生)和类固醇生成中的局部调节作用，为鱼类睾丸和卵巢功能的旁分泌调节提供了新的信息。我们最近的发现表明，促性腺激素抑制激素(GnIH)和甲状腺激素是影响鱼类繁殖和生长综合控制的关键因素。这项拟议的研究将建立在我们过去工作的基础上，并集中在我的团队同时进行的三个主要研究领域：(1)甲状腺激素和GnIH的作用，与脑神经激素和性腺激素协同工作，调节垂体促性腺激素和生长激素的产生；(2)甲状腺激素和GnIH的作用，与垂体和性腺激素协同工作，调节卵巢和睾丸的发育和分化。(3)能量支持和维持长时间繁殖和生长所需的代谢途径的变化。这些领域的拟议研究将旨在满足以下主要研究目标：*1.了解促性腺激素和生长激素产生的综合控制*2.了解生殖和生长阶段睾丸和卵巢发育和功能的综合调节*3.阐明维持生殖和生长的代谢调节*我们将使用最先进的设施和创新的实验方法来研究拟议的目标。这些项目将被用作在比较内分泌学的广泛领域培训HQP的工具，将涉及三名博士和两名硕士学生以及几名本科生、访问学生和科学家。这项拟议的研究将加深我们对鱼类生殖和生长的综合相互控制以及相关代谢变化的理解，并为睾丸和卵巢发育和功能的综合调控提供重要信息。这些结果将与所有脊椎动物物种相关，因为它提供了对生殖和生长之间微妙平衡的调节机制的洞察。此外，拟议的研究将通过提高我们评估在水生生态系统中具有激素样活动的环境污染物的不利影响的能力，在水产养殖和环境毒理学方面具有翻译潜力。