Multifactorial regulation of reproduction and gonadal development in fish

多年来，我的研究目标一直是通过研究促性腺激素和生长激素产生的大脑调节来了解鱼类生殖和生长的激素控制。我们对下丘脑促性腺激素释放激素调节垂体促黄体激素、促卵泡激素和生长激素产生的机制的理解做出了贡献。我们还提供了新的信息旁分泌调节睾丸和卵巢功能的鱼类性腺激素的配子发生（卵子和精子的生产）和类固醇激素的调节的局部影响进行调查。我们最近的研究结果表明，促性腺激素抑制激素（GnIH）和甲状腺激素是影响鱼类繁殖和生长的综合控制的关键因素之一。本研究将以我们过去的工作为基础，集中在三个主要的研究领域：（1）甲状腺激素和GnIH的作用，与脑神经激素和性腺激素协同作用，调节垂体促性腺激素和生长激素的相互控制;（2）甲状腺激素和GnIH与垂体和性腺激素协同作用，调节卵巢和睾丸发育和分化。(3)代谢途径的变化需要大力支持和维持长时间的繁殖和生长。在这些范畴内进行的拟议研究，会符合下列主要研究目标： 1.了解促性腺激素和生长激素产生的综合控制 2.了解生殖和生长阶段睾丸和卵巢发育和功能的综合调节 3.阐明新陈代谢的调节以维持生殖和生长 我们将使用最先进的设施和创新的实验方法来研究拟议的目标。这些项目将被用作比较内分泌学广泛领域的HQP培训工具，并将涉及三名博士和两名硕士学生以及几名本科生，访问学生和科学家。拟议的研究将提高我们对鱼类生殖和生长的综合相互控制以及相关代谢变化的理解，并提供有关睾丸和卵巢发育和功能综合调节的重要信息。这些结果将与所有脊椎动物物种相关，通过提供对生殖和生长之间微妙平衡调节机制的深入了解。此外，拟议的研究将在水产养殖和环境毒理学方面具有转化潜力，提高我们评估水生生态系统中具有类似鱼类活动的环境污染物的不利影响的能力。

My research objective over many years has been to understand hormonal control of reproduction and growth in fish by studying brain regulation of gonadotropin and growth hormone production. We have contributed to the understanding of mechanisms by which hypothalamic gonadotropin-releasing hormone regulates pituitary luteinizing hormone, follicle stimulating hormone and growth hormone production. We have also provided novel information on paracrine regulation of testicular and ovarian function in fish by investigating the local effects of gonadal hormones in the regulation of gametogenesis (egg and sperm production) and steroidogenesis. Our recent findings suggest that gonadotropin-inhibitory hormone (GnIH) and thyroid hormones are among the key factors that influence the integrated control of reproduction and growth in fish. The proposed study will build on our past work and focus on three primary research areas conducted simultaneously in my group: (1) The role of thyroid hormones and GnIH, working in concert with brain neuro-hormones and gonadal hormones, to regulate reciprocal control of pituitary gonadotropins and growth hormone production; (2) The role of thyroid hormones and GnIH, working in concert with pituitary and gonadal hormones, to regulate ovarian and testicular development and differentiation. (3) Changes in metabolic pathways required to energetically support and sustain prolonged periods of reproduction and growth. The proposed studies in these areas will be designed to meet the following major research objectives: 1. Understand the integrated control of gonadotropin and growth hormone production 2. Understand the integrated regulation of testicular and ovarian development and function during reproductive and growth phases 3. Elucidate the regulation of metabolism to sustain reproduction and growth We will use state-of-the-art facilities and innovative experimental approaches to investigate the proposed objectives. These projects will be used as a vehicle for the training of HQP in the broad areas of comparative endocrinology, and will involve three PhD and two MSc students as well as several undergraduate students, visiting students and scientists. The proposed research will enhance our understanding of the integrated reciprocal control of reproduction and growth and associated changes in metabolism in fish, as well as providing important information on integrated regulation of testicular and ovarian development and function. The results will be relevant to all vertebrate species by providing an insight into the mechanisms by which delicate balance between reproduction and growth is regulated. Furthermore, the proposed studies will have translational potential in aquaculture and environmental toxicology by improving our ability to assess adverse impacts of environmental contaminants with hormone-like activities in the aquatic ecosystem.